| 甘草离体培养物中总黄酮提取工艺的优化 |
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| 作者姓名： | 葛淑俊 李广敏 马峙英 王文全 安金翠 孟义江 |
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| 作者单位： | 河北农业大学 保定071001(葛淑俊，马峙英，安金翠，孟义江)，河北省农林科学院 石家庄050051(李广敏)，北京中医药大学 北京100102(王文全) |
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| 基金项目： | 河北省自然科学基金项目“甘草有效成分甘草酸合成的细胞培养技术研究”（C2004000318）;河北省教育厅项目“甘草有效成分甘草酸合成的细胞培养技术研究”（2004438）. |
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| 摘 要： | 【研究目的】对甘草离体培养物中总黄酮提取和测定工艺进行优化,以建立在室内筛选高产细胞系和试管苗株系的方法;【方法】采用超声波提取技术,对提取液、浸泡时间、提取温度等进行优化;【结果】将样品以75%甲醇浸泡6h后在32℃下超声提取两次,比色法测定总黄酮含量,加样回收率为95.3%～101.4%,相对标准偏差为1.61%。测得甘草初代愈伤组织中总黄酮含量为0.0012%～0.05%,子叶愈伤组织调控后含量高达0.08%,试管苗中总黄酮含量为1.04%～1.31%,移栽后高达4.5%。【结论】此工艺准确度较高、稳定性好、简便可行,为优良细胞系的筛选和试管苗的早期鉴定奠定了技术基础。
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| 关 键 词： | 甘草 离体培养物 总黄酮 超声提取 |
Extraction and Content Determination of Total Flavonoids of Cultures of Glycyrrhiza uralensis Fisch. |
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| Authors: | Ge Shujun Li Guangmin Ma Zhiying Wang Wenquan An Jincui Meng Yijiang |
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| Institution: | A gricultural University of Hebei, Baoding 071001; 2Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051; 3 Beijing University of Chinese Medicine, Beijing 100102 |
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| Abstract: | objective] to optimize the extraction and determination method of the total flavonoids thus to screen the cell clones and plantlets with high contents,method] the ultrasonic technique was used and several treatments were tried and screened,result] the best condition was marinated 6 hours with 75% carbinol then ultrasonic extracted twice.And then the content was determined with colorimetry from cultures of Glycyrrhiza uralensis Fisch.The result showed the recovery rate was between 95.3% and 101.4% and the RSD was 1.61%.The total flavonoids of the initial calli was between 0.0012% and 0.05%,the improvement cotyledonary calli was up to 0.08%,the contents of the plantlets were between 1.04% and 1.31%,and after being transplanted was up to 4.5%.conclusion] The rate of veracity of this method was high and this provided the determination of Glycyrrhiza cultures flavonoids products a feasible method,and those data offered a credible academic gist for authenticate of the experiment resource. |
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| Keywords: | Glycyrrhiza uralensis Fisch Cultures Total flavonoids Ultrasonic extraction |
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