| 一种快速低廉的PCR探针标记方法 |
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| 作者姓名： | 许本波 谢伶俐 柴友荣 李加纳 田志宏 |
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| 作者单位： | 长江大学生命科学学院 湖北荆州434025(许本波，谢伶俐，田志宏)，西南大学农学与生物科技学院 重庆400716(柴友荣，李加纳) |
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| 基金项目： | 国家自然科学基金重点项目＂甘蓝型油菜优质高效黄籽性状的遗传基础研究＂（30330400）. |
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| 摘 要： | 【研究目的】探针标记技术是分子生物学和基因工程研究中常用实验技术,需要发展高效、快速、低成本、安全、简捷的DNA探针标记方法;【方法】利用普通的Taq酶进行PCR探针标记,电泳和Southern杂交检测探针标记结果;【结果】电泳检测发现标记产物在电泳时相对非标记的对照表现明显滞后,表明探针标记成功;电泳条带清晰明亮,表明探针标记效率高;Southern杂交条带清晰,说明该方法标记的探针可用于Southern等分子杂交实验;【结论】利用普通的Taq酶进行PCR探针标记,是一种低成本、高效、快速的DNA探针标记方法。
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| 关 键 词： | DNA探针标记 Taq DNA PCR标记 |
A Rapid and Twopenny PCR Labefing Method |
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| Authors: | Xu Benbo Xie Lingli Chai Yourong Li Jian Tian Zhihong |
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| Institution: | CoUege of Life Science, YangLze University, Jingzhou Hubei 434025; 2College of A gronomy and Biotechnology, Southwest University, Chongqing 400716 |
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| Abstract: | OBJECTIVE]DNA labeling technology is important for research of molecular and genetic engineering,So it is important to develop a high-efficiency,rapid,low-cost,safe,simple and convenient method for labeling DNA;METHOD]Based on PCR principle,DNA was labeled using Taq DNA Polymerase and the result was detected by electrophoresis and southern-blot.RESULTS]The electrophoresis results indicated the product labeled was lag than control DNA,which improved that the label succeed;The bright and clear strip indicated the labeling efficiency was high;Cross strip in southern-blot was clear,which proved the product labeled by the method can be used in molecular hybridization.CONCLUSION]PCR labeling using Taq DNA polymerase is a low-cost,high-efficiency and rapid DNA labeling method. |
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| Keywords: | Labeling DNA Taq DNA polymerase PCR labeling |
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