| 猪圆环病毒Ⅱ型PCR检测方法的研究 |
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| 作者姓名： | 王宪文 刘兴友 梁美兰 郑玉姝 刘丽艳 |
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| 作者单位： | 河南农业大学，河南农业大学，河南农业大学，河南农业大学，河南农业大学，河南农业大学，河南农业大学 郑州450002，郑州450002，郑州450002，郑州450002郑州牧业工程高等专科学校，郑州450011，郑州450002，郑州450002，郑州450002 |
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| 基金项目： | 河南省杰出人才创新基金＂优质牧草专用高效微肥的研制与开发＂项目（0521001700）资助. |
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| 摘 要： | 采用叶面喷施的方法,研究了不同施钴水平对紫花苜蓿鲜草产量和营养品质的影响。结果表明:喷施硫酸钴显著(P<0.05)提高了苜蓿鲜草产量,其中施钴量为300mg/kg的处理效果最好,比对照CK产量可以提高25%。同时显著能够提高苜蓿叶茎比。不同施钴水平对苜蓿营养成分的含量影响不同,苜蓿粗蛋白及磷含量随随钴量的增加而增加,而对苜蓿粗纤维、粗灰分和无氮浸出物含量没有显著的影响,粗脂肪则随着施钴量的增加而逐渐降低。不同施钴水平对紫花苜蓿营养物质产量影响不同,其中粗蛋白、粗灰分、磷和无氮浸出物产量均以Co2处理300mg/kg最高,都要显著高于对照,粗纤维、钙产量也以Co2处理最高,但与CK没有显著差异,粗脂肪产量则以Co1处理100mg/kg效果最好。
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| 关 键 词： | 紫花苜蓿 硫酸钴 鲜草产量 品质 |
Detection of Procine Circovirus Type 2 by PCR Technique |
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| Authors: | Wang Xianwen Liu Xingyou Liang Meilan Zheng Yushu Liu Liyan |
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| Institution: | Henan Agricultural University, Zhengzhou 450002; Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011 |
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| Abstract: | The paper studied the effect of different level of cobalt sulfate on fresh grass yield and nutrient quality of alfalfa by leaf surface spurting.The result indicated that,Spurting cobalt sulfate could remarkably(P < 0.05)increase the fresh grass yield of alfalfa and the leaf stem ratio(LS).The effect of the concentration of 300 mg/kg treatment is best,in compared to CK treatment,the fresh grass yield increased by 25%.Different levels of cobalt sulfate affect the nutrient content differently.The contents of crude protein(CP)and P was increased with the applied Co amount increased,while there was on significantly different on content of crude fiber(CF),nitrogen free extract(NFE),crude ash(CA),and the content of crude fat(CF)was decreased with the applied Co amount increased.When the Co concentration was 300 mg/kg,the yield of crude protein(CP),crude ash(CA),P,NFE was reach max.,among the all of treatment,and significantly higher than that of CK treatment,the yield of crude fiber and Ca of treatment Co2 was reach max.,but there were no significantly different to CK.and crude fat was Co1 treatment. |
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| Keywords: | Alfalfa (Medicaco sativa L ) Cobalt sulfate Fresh Grass Yield Quality |
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