| 大肠埃希氏菌表面菌毛黏附因子多价菌毛疫苗的研制 |
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| 引用本文： | 李鹏,王家乡,程碧军.大肠埃希氏菌表面菌毛黏附因子多价菌毛疫苗的研制[J].湖北农业科学,2006,45(5):643-646. |
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| 作者姓名： | 李鹏 王家乡 程碧军 |
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| 作者单位： | 1. 长江大学动物科学学院,湖北,荆州,434025
2. 华中农业大学动物医学院,武汉,430070 |
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| 基金项目： | 湖北省科技厅科技攻关项目 |
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| 摘 要： | 为了预防仔猪黄痢,选取ETEC野生分离菌株HN2001(K88ab)、HN2002(K88ac)、HN2003(K88ad)、HN2004(K99)、HN2005(987p)、HN2006(F41)培养后用低温磁力搅拌法提取到菌毛,制成5批多价菌毛混合油乳剂灭活苗,实验室试验结果表明,5批多价灭活疫苗对小白鼠的平均保护率达95.0%,为进一步进行田间试验提供了理论依据,并将为生产上防治仔猪黄痢提供一种保护范围更加全面的生物制品.
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| 关 键 词： | 大肠埃希氏菌 菌毛黏附因子 多价菌毛疫苗 研制 |
| 文章编号： | 0439-8114(2006)05-0643-03 |
| 收稿时间： | 05 8 2006 12:00AM |
| 修稿时间： | 2006年5月8日 |
Development of the Multivalent Pili Oil-emulsion Vaccine of Escherichia coli Fimbriae Adherence Factor |
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| LI Peng,WANG Jia-xiang,CHENG Bi-jun.Development of the Multivalent Pili Oil-emulsion Vaccine of Escherichia coli Fimbriae Adherence Factor[J].Hubei Agricultural Sciences,2006,45(5):643-646. |
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| Authors: | LI Peng WANG Jia-xiang CHENG Bi-jun |
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| Institution: | 1.College of Animal Science,Yangtze University, Jingzhou 434025, Hubei, China, 2.College of Animal Medicine, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | In order to prevent yellow scour of the newborn piglets,wild strains HN2001(K88ab),HN2002(K88ac),HN2003(K88ad),HN2004(K99),HN2005(987p) and HN2006(F41) were separated and Selected to cultivate.The pili of the cultivated strains was purified with whisking way by bar magnet in low temperature,and 5 batches of multivalent pili oil-emulsion vaccines were obtained.The results of protection experiments on mice showed that 95%(38/40) of mice were protected,which provided the theory basis for further field experiment and a kind of biological preparation to prevent and control the newborn pig diarrhea in practice. |
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| Keywords: | K88-K99-987p-F41 multivalent pili oil-emulsion vaccine development |
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