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| 猪食道口线虫ITS-1和lTS-2 rDNA的PCR-SSCP分析 | |
| 引用本文： | 林瑞庆,张新高,胡友兰,李国清,翁亚彪,宋慧群,朱兴全.猪食道口线虫ITS-1和lTS-2 rDNA的PCR-SSCP分析[J].中国预防兽医学报,2006,28(3):323-326. |
| 作者姓名： | 林瑞庆 张新高 胡友兰 李国清 翁亚彪 宋慧群 朱兴全 |
| 作者单位： | 1. 华南农业大学,兽医学院,广东,广州,510642 2. 深圳市南山区动物防疫监督所,广东,深圳,518051 3. 重庆市动物卫生监督总站,重庆,401147 |
| 基金项目： | 国家杰出青年科学基金;教育部优秀青年教师资助计划 |
| 摘 要： | 以采自我国不同地区猪体的食道口线虫虫株为研究对象,PCR扩增出ITS-1和ITS-2序列片段,然后采用单链构象多态性(SSCP)方法分析PCR产物,对不同地区食道口线虫进行分子鉴定。所有样品经SSCP分析显示两种带型,第一种为有齿食道口线虫带型,另一种为未定种食道口线虫带型。代表性样品的测序结果表明,未定种食道口线虫带型的样品为四棘食道口线虫。本研究在国际上首次报道了中国猪四棘食道口线虫的ITS序列,并建立了区分有齿食道口线虫和四棘食道口线虫的PCR-SSCP方法,从而为食道口线虫的分子生物学的进一步研究奠定了基础。 |
| 关 键 词： | 食道口线虫 单链构象多态性(SSCP) |
| 文章编号： | 1008-0589(2006)03-0323-04 |
| 修稿时间： | 2005年3月31日 |
Analysis of the ITS-1 and ITS-2 rDNA of Oesophagostomum from pig by PCR-SSCP |
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| LIN Rui-qing,ZHANG Xin-gao,HU You-lan,LI Guo-qing,WENG Ya-biao,SONG Hui-qun,ZHU Xing-quan.Analysis of the ITS-1 and ITS-2 rDNA of Oesophagostomum from pig by PCR-SSCP[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(3):323-326. | |
| Authors: | LIN Rui-qing ZHANG Xin-gao HU You-lan LI Guo-qing WENG Ya-biao SONG Hui-qun ZHU Xing-quan |
| Abstract: | Genetic variation in the first(ITS-1) and second(ITS-2) internal transcribed spacers(ITS) of ribosomal DNA(rDNA) among Oesophagostomum samples from pigs and different geographical locations in China was examined by single-strand conformation polymorphism(SSCP) analysis.The ITS-1 and ITS-2 were amplified by PCR separately from individual nematodes and the amplicons were analyzed by SSCP.SSCP analyses showed that all Oesophagostomum samples could be classified into two groups: Oesophagostomum dentatum and Oesophagostomum sp..Subsequently,Oesophagostomum sp.was identified as Oesophagostomum quadrispinulatum by sequencing.PCR-SSCP analysis allowed the identification and differentiation of O.dentatum and O.quadrispinulatum,and it was the first time that the complete sequence of ITS rDNA of O.quadrispinulatum from China was reported.The results of this study provided a foundation for the further study of Oesophagostomum. |
| Keywords: | ITS-1 ITS-2 |
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