| 二株广西猪繁殖与呼吸综合征流行毒M基因的克隆和序列分析 |
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| 引用本文： | 罗廷荣,唐璋辉,蒋小红,孙文博,刘芳,陆芹章,黄伟坚,余克伦.二株广西猪繁殖与呼吸综合征流行毒M基因的克隆和序列分析[J].中国预防兽医学报,2004,26(2):90-93. |
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| 作者姓名： | 罗廷荣 唐璋辉 蒋小红 孙文博 刘芳 陆芹章 黄伟坚 余克伦 |
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| 作者单位： | 广西大学,动物科学技术学院,广西,南宁,530005 |
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| 摘 要： | 广西一些养殖场频繁发生以母猪流产、死胎、木乃伊等为特征的流行性传染病,怀疑为猪繁殖与呼吸综合征病毒(PRRSV)所致.利用针对PRRSV的N基因的特异性引物P1和P2,通过RT-PCR技术对分别从广西贵港市(GXGG)和南宁市(GXNN)收集到的可疑病料进行检测,结果两份为阳性.合成针对M基因的引物M1和M2,分别扩增了GXGG和GXNN两株PRRSV的M基因,并进行克隆和测序,得到长582个核苷酸的目的基因片段.应用DNAStar序列分析软件对所测的两个广西毒株与国内外已发表的ATCC VR-2332、LV和CH-la毒株进行同源性比较.分析表明,GXGG与ATCC VR-2332、LV、CH-la的核苷酸同源性分别为95.6%、69.5%、97.7%;GXNN与ATCC VR-2332、LV、CH-la的核酸同源性分别为94.9%、69.5%、97.3%.对推定的氨基酸序列进行了比较,GXGG与ATCC VR-2332、LV、CH-la的氨基酸同源性分别为97.7%、80.5%、97.7%;GXNN与ATCC VR-2332、LV、CH-la的氨基酸同源性分别为98.3%、79.9%、97.1%.说明广西流行毒株与ATCC VR-2332和CH-la的同源性很高,而与LV毒株的同源性很低.从本研究构建的系统发育树分析,广西流行的PRRSV与ATCC VR-2332株的亲缘关系比较密切.
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| 关 键 词： | M基因 同源性 |
| 文章编号： | 1008-0589(2004)02-0090-04 |
| 修稿时间： | 2003年6月16日 |
Cloning and sequence analysis of M gene of two PRRSV strains epidemic in Guangxi |
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| LUO Ting-rong,TANG Zhang-hui,JIANG Xiao-hong,LIU Fang,LU Qin-zhang,HUANG Wei-jian,YU Ke-lun.Cloning and sequence analysis of M gene of two PRRSV strains epidemic in Guangxi[J].Chinese Journal of Preventive Veterinary Medicine,2004,26(2):90-93. |
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| Authors: | LUO Ting-rong TANG Zhang-hui JIANG Xiao-hong LIU Fang LU Qin-zhang HUANG Wei-jian YU Ke-lun |
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| Abstract: | In recent, some pig farms in Guangxi occur frequently an infectious disease,which characters are abortion,death of embryo and mummified fetuses.Pathogen of the disease is considered as porcine reproductive and respiratory syndrome virus(PRRSV).With a specific N gene primers of PRRSV,we identified that the samples collected from these famers in Nanning and Guigang were positive by RT-PCR Based on it,we designed two primers M1 and M2.According to the sequence of M gene.The fragments had been successfully amplified from RNA of PRRSV from GXGG and GXNN samples.The amplified fragments were cloned and sequenced.The length of M gene fragments is 582 base pair.Comparison of the homology among GXGG,GXNN,ATCC VR-2332,Lelystad virus and CH-la strain by DNAStar software,the results showed that the homology of nucleotide siquences of nucleotide sequences of GXGG and ATCC VR-2332,Lelystad virus,CH-la were 95.6 %,69.5 %,97.7 %,respectively.and the homology of nucleotide sequences or GXNN and ATCC VR-2332,Lelystad virus,CH-la were 94.9 %,69.5 %,97.3 %,respectively.Comparison of the deduced amino acid sequences showed that strains GXGG and ATCC VR-2331,Lelystad Virus,CH-la were 97.7 %,80.5 %,97.7 %,respectively.Those analysis showed that the homologies among Guangxi,ATCC VR-2332 and CH-la strain were very high,but very low between Guangxi strain and ATCC VR-2332,CH-la or Lelystad virus.And the phylogenetic tree revealed that Guangxi strains perhaps have a close relation to ATCC VR-2332 and CH-la. |
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| Keywords: | PRRSR |
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