| 猪囊尾蚴(Cvsticercus cellulosae)抗原制剂的PAGE圆盘电泳分析 |
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| 引用本文： | 车丽美,周源昌.猪囊尾蚴(Cvsticercus cellulosae)抗原制剂的PAGE圆盘电泳分析[J].东北农业大学学报,1991(2). |
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| 作者姓名： | 车丽美 周源昌 |
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| 作者单位： | 东北农学院兽医寄生虫学教研室，东北农学院兽医寄生虫学教研室 |
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| 摘 要： | 用聚丙烯酰胺凝胶圆盘电泳法对猪囊尾蚴的全囊虫、囊液、头节的粗提原(CW_1,CF_1,CS_1),超速离心上清抗原(CW_2,CF_2,CS_2),及葡聚糖凝胶柱(Sephadex G—150)层析提纯抗原(CW_3,CF_3,CS_3)进行了分析。结果表明,CW_1,CF_1和CS_1抗原分别显示12,5和9条氨基酸染色区带;CW_2,CF_2和CS_2抗原分别显示10,3和5条区带;而CW_3和CF_3抗原分别显示2和3条区带。由此可见,提纯后的抗原组分较纯。通过脂蛋白染色预电泳法和阿尔新兰染色法证明,囊液抗原含有丰富的蛋白质,同时含有糖蛋白和脂蛋白成分,而头节抗原则未出现糖蛋白和脂蛋白染色区带。
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| 关 键 词： | 猪 聚丙烯酰胺圆盘电泳法 囊尾蚴抗原 |
ANALYSIS ON CYSTICERCUS CELLULOSAEANTIGENS BY POLYACRILAMIDE GEL ELECTROPHORESIS |
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| Che Limei,Zhou Yuanchang Northeast Agricultural collgeg,Harbin ,China.ANALYSIS ON CYSTICERCUS CELLULOSAEANTIGENS BY POLYACRILAMIDE GEL ELECTROPHORESIS[J].Journal of Northeast Agricultural University,1991(2). |
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| Authors: | Che Limei Zhou Yuanchang Northeast Agricultural collgeg Harbin China |
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| Institution: | Che Limei,Zhou Yuanchang Northeast Agricultural collgeg,Harbin 150030,China |
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| Abstract: | The Cysticercus cellulosae antigens were analysed by polyacrilamide gel elcctrophoresis.CW_1, CW_2 and CW_3 antigens showed 12, 10 and 2 bands of protein respectively; CF_1,CF_2 and CF_3 antigens showed 5, 3 and 3 bands of protein respectively. From this it could beseen that CW_3 and CF_2 antigens were purer than the crude antigens, but their componentswere complex though. Both CF and CW antigens showed a band of Alcian blue or Sudanblack. So they contain glycoprotein and lipoprotein. CS antigen did not show any band ofAlcian blue or Sudan black. |
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| Keywords: | polyacrilamide gel electrophoresis Cysticercus cellulosae antigen |
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