| 新城疫病毒在不同的细胞上增殖特性的研究 |
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| 引用本文： | 古长庆,金宁一,金扩世,李萍,殷震.新城疫病毒在不同的细胞上增殖特性的研究[J].黑龙江畜牧兽医,2000(10):5-6. |
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| 作者姓名： | 古长庆 金宁一 金扩世 李萍 殷震 |
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| 作者单位： | 解放军军需大学研究所,吉林长春 |
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| 基金项目： | 国家自然科学基金，39893290-4-3， |
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| 摘 要： | 研究新城疫病毒（Ｎｅｗｓｃａｓｔｌｅ ｄｉｓｅａｓｅ Ｖｉｒｕｓ，ＮＤＶ）可，弱毒株在不同的传代细胞的生物学特性。Ｆ４８Ｅ８强毒株在ＢＨＫ、Ｖｅｒｏ和ＣＥＦ中都能增殖并产生细胞病变，而Ｖ４生弱毒株不能在ＢＨＫ和Ｖｅｒｏ中增殖，当ＭＥＭ含１０ｕｇ／ｍＬ胰蛋白酶时，ＮＤＶ弱毒株在Ｖｅｒｏ中能较好地复制，将Ｖ４株在Ｖｅｒｏ中传代培养后，Ｖｅｒｏ中传代的Ｖ４毒株的ＨＡ较低，但毒力增强，ＢＨＫ中传代的Ｖ４毒
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| 关 键 词： | 新城疫病毒 疫苗 细胞病变 传代细胞 |
| 文章编号： | 1004-7034(2000)10-0005-02 |
| 修稿时间： | 2000年1月15日 |
Study on Proliferation Characteristics of Newscastle Disease Virus on the different Cells |
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| GU Chang-qing,JIN Ning-yi,JIN Kuang-shi,et al.Study on Proliferation Characteristics of Newscastle Disease Virus on the different Cells[J].Heilongjiang Animal Science And veterinary Medicine,2000(10):5-6. |
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| Authors: | GU Chang-qing JIN Ning-yi JIN Kuang-shi |
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| Abstract: | The experiment was taken in order to study the biological characteristics of Newscastle Disease Virus(NDV) strong and weak strain on BHK cells. The results showed that F 48 E 8 could proliferate in BHK cell,Vero cell and CEF cell, and make the cells pathological changes, but the V4 couldn't proliferate in BHK cell and Vero cell. When the concentration of trypsinogen is 10ug/ml in MEM, weak NDV strain could copy in BHK cell and Vero cell. Cultivated V4 strain in Vero cell, the HA of V4 in Vero cell was lower, virulence became strong, the HA and virulence of V4 in BHK cell were both increase , equaled to the virulence and HA on chicken embryo. It would be approved that immunogen of V4 proliferated in BHK cell was better after further study, it is possible use BHK cell series to replace chicken embryo to proliferate V4 as virus antigen of vaccine. The characteristics of NDV vaccine produced using BHK cell series were no risk of spread illrn stalk rot diease; pathogen; KCl; ZnSO 4; Si |
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| Keywords: | Newscastle Disease Virus Vaccine Pathological Changes |
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