| 羊附红细胞体抗原的分析及初步应用 |
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| 引用本文： | 张守发,贾立军.羊附红细胞体抗原的分析及初步应用[J].河南农业科学,2007(11):112-114. |
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| 作者姓名： | 张守发 贾立军 |
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| 作者单位： | 延边大学,农学院,吉林,龙井,133400 |
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| 摘 要： | 将提取的羊附红细胞体抗原进行SDS-PAGE电泳及免疫印迹分析,并以此抗原为诊断抗原,用ELISA方法对64份羊的血清样本进行了初步检测。结果表明,56kDa和26kDa的羊附红细胞体抗原具有较好的免疫性,而且不与羊附红细胞体阴性血清和羊支原体病阳性血清发生交叉反应,具有较好的特异性;ELISA检测的64份血清样本的阳性率为66.7%,比涂片镜检高22.9个百分点。
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| 关 键 词： | 羊附红细胞体 抗原 免疫印迹 ELISA |
| 文章编号： | 1004-3268（2007）11-0112-03 |
| 修稿时间： | 2007-05-12 |
Analysis and Application of E. Ovis Antigen |
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| ZHANG Shou-fa,JIA Li-jun.Analysis and Application of E. Ovis Antigen[J].Journal of Henan Agricultural Sciences,2007(11):112-114. |
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| Authors: | ZHANG Shou-fa JIA Li-jun |
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| Institution: | Agricultural College of Yanbian University,Longjing 133400 |
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| Abstract: | The antigen trait and immunogenicity of E.ovi were analyzed using SDS-PAGE and western blotting,then the E.ovis antigen was used as a diagnostic antigen to detect 64 serum samples by ELISA.The results showed that the 56kD and 26kD antigens had better immunogenicity and could not react with the sheep positive serum of Mycoplasma pneumonia and negative serum of E.ovis.Of the 64 samples detected,the positive rate was 66.7%(42/64),22.9% higher than that of direct microscopic examinerion. |
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| Keywords: | E ovis Antigen Western blotting ELISA |
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