| 卡那霉素对川芎愈伤组织诱导和生长的影响 |
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| 引用本文： | 蒋卫东,唐琳,杨苍劲,马逾英,蒋桂华,陈放.卡那霉素对川芎愈伤组织诱导和生长的影响[J].河南农业科学,2007,55(11):85-87. |
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| 作者姓名： | 蒋卫东 唐琳 杨苍劲 马逾英 蒋桂华 陈放 |
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| 作者单位： | 1. 四川大学,生命科学学院,四川,成都,610064
2. 成都中医药大学,药学院,四川,成都,611137 |
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| 摘 要： | 以川芎(Ligusticum chuanxiong Hort.)的根、茎、叶及其对应的愈伤组织为试验材料,研究不同浓度的卡那霉素对其诱导和生长的影响。不同的外植体对卡那霉素的敏感度不同,根的敏感度高于茎,茎的敏感度高于叶片。方差分析和总体观察的结果表明,在川芎的遗传转化中,卡那霉素的最佳抗性筛选浓度范围为20~40 mg/L。
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| 关 键 词： | 卡那霉素 川芎 愈伤组织 方差分析 遗传转化 |
| 文章编号： | 1004-3268（2007）11-0085-03 |
| 修稿时间： | 2007-05-11 |
Effects of Kanamycin on the Induction and Growth of Ligusticum chuanxiong Hort. Callus |
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| JIANG Wei-dong,TANG Lin,YANG Cang-jin,MA Yu-ying,JIANG Gui-hua,CHEN Fang.Effects of Kanamycin on the Induction and Growth of Ligusticum chuanxiong Hort. Callus[J].Journal of Henan Agricultural Sciences,2007,55(11):85-87. |
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| Authors: | JIANG Wei-dong TANG Lin YANG Cang-jin MA Yu-ying JIANG Gui-hua CHEN Fang |
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| Institution: | 1. College of Life Sciences, Sichuan University, Chengdu 610064,China; 2. School of Pharmacology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China |
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| Abstract: | The roots,stems,leaves and their correspinding calli of Ligusticum chuanxiong Hort.were used as material to study the effects of different concentrations of Kanamycin on their growth.The sensitivity of explants to Kanamycin was different,with a rank of root 〉stems〉leaves.Variance analysis and overall observation indicated that the optimum Kanamycin concentration for resistance selection was 20-40mg/L. |
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| Keywords: | Kanamycin Ligusticum chuanxiong Hort Callus Analysis of variance(ANOVA) Genetic transformation |
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