Spontaneous Adenosquamous Carcinoma with Rapid Growth and EMT-like Changes in the
Mammary Gland of a Young Adult Female BALB/c Mouse |
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| Authors: | Katsumi Takaba Teruyoshi Imada Shigehisa Katsumata Hiroshi Okumura Sachiko Iwamoto Yui Suzuki Minami Imaizumi Kensuke Myojo Chie Takada Naoya Kimoto Koji Saeki Itaru Yamaguchi |
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| Institution: | 1. Toxicological Research Laboratories, Kyowa Hakko Kirin Co., Ltd., 1188 Shimotogari, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8731, Japan;2. Laboratory Animal Services, Fuji Research Park, Kyowa Hakko Kirin Co., Ltd., 1188 Shimotogari, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8731, Japan |
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| Abstract: | This study histopathologically and immunohistochemically investigated a spontaneously
occurring single mass subcutaneously located in the left lower abdomen of a female
BALB/cAJcl−nu/+ mouse at 10 weeks of age. The mass was about 20 × 15 × 10 mm
in size after formalin fixation; nevertheless, it was not detected by clinical observations at
9 weeks of age. H&E staining revealed the tumor origin was epithelial and probably arose
from the mammary gland, and the tumor cells demonstrated a squamous, acinar or polyhedral/basal
pattern. A cell kinetics analysis revealed that many of the tumor cells of the squamous, acinar
or polyhedral/basal component were positive for PCNA and cyclin D1, although there were a few
of TUNEL-positive tumor cells in all of the components. An epithelial/mesenchymal analysis
demonstrated that most of the tumor cells of the squamous and acinar components contained
keratin and E-cadherin; however, most of the tumor cells of the polyhedral/basal component were
less or very weakly positive for these markers. The tumor cells of the squamous component were
negative for vimentin and SMA; however, many of the tumor cells of the polyhedral/basal
component exhibited vimentin. In addition, expression of SMA was confirmed in some tumor cells
of the acinar and basal components. Based on the microscopic and immunohistochemical
characterizations, the tumor was diagnosed to be adenosquamous carcinoma that originated from
the mammary gland with rapid growth, and the tumor cells demonstrated epithelial-mesenchymal
transition-like changes. |
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| Keywords: | adenosquamous carcinoma rapid growth mouse immunohistochemistry |
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