Acute Phase Pulmonary Responses to a Single Intratracheal Spray Instillation
of Magnetite (Fe3O4) Nanoparticles in Fischer 344
Rats |
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| Authors: | Yukie Tada Norio Yano Hiroshi Takahashi Katsuhiro Yuzawa Hiroshi Ando Yoshikazu Kubo Akemichi Nagasawa Akio Ogata Dai Nakae |
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| Institution: | 1. Departments of Environmental Health and Toxicology, Tokyo Metropolitan Institute of Public Health, 3-24-1 Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan;2. Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan |
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| Abstract: | Iron nanomaterials are of considerable interest for application to
nanotechnology-related fields including environmental catalysis, biomedical imaging, drug
delivery and hyperthermia, because of their superparamagnetic characteristics and high
catalytic abilities. However, information about potential risks of iron nanomaterials is
limited. The present study assessed pulmonary responses to a single intratracheal spray
instillation of triiron tetraoxide nanoparticles (magnetite) in rats. Ten-week-old male
and female Fischer 344 rats (n=5/group) were exposed to a single intratracheal spray
instillation of 0 (vehicle), 5.0, 15.0 or 45.0 mg/kg body weight (BW) of magnetite. After
14 days, the rats were sacrificed, and biological consequences were investigated. The lung
weights of the 15.0 and 45.0 mg/kg BW male and female groups were significantly higher
than those of the control groups. The lungs of treated rats showed enlargement and black
patches originating from the color of magnetite. The typical histopathological changes in
the lungs of the treated rats included infiltration of macrophages phagocytosing
magnetite, inflammatory cell infiltration, granuloma formation and an increase of goblet
cells in the bronchial epithelium. The results clearly show that instilled magnetite
causes foreign body inflammatory and granulating lesions in the lung. These pulmonary
responses occur in a dose-dependent manner in association with the increase in lung
weight. |
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| Keywords: | magnetite Fe3O4 nanoparticles lung intratracheal spray instillation Fischer 344 rat |
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