Intracytoplasmic Sperm Injection in Livestock Species: An Update |
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| Authors: | E García-Roselló E García-Mengual P Coy J Alfonso MA Silvestre |
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| Institution: | Dpto. Medicina y Cirugía Animal, Facultad de Ciencias Experimentales y de la Salud, Universidad CEU-Cardenal Herrera, Edificio Seminario, Moncada;;Centro de Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias, Segorbe (Castellón);;Dpto. Fisiología, Facultad de Veterinaria, Universidad de Murcia, Murcia;;Instituto de Medicina Reproductiva (IMER), Valencia, Spain |
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| Abstract: | Intracytoplasmic sperm injection (ICSI) is a powerful technique in the field of assisted reproduction (ART) and provides exciting opportunities for studying the basic mechanisms of fertilization and early embryo development. Nevertheless, its application in agriculture and conservation biology has been greatly hampered by the low success rate reported for this method in respect of economically important species. Specifically, the rates of blastocyst formation and live newborn are greatly reduced when zygotes are generated by ICSI. Except for humans, ICSI remains a low efficiency technology in comparison with alternatives such as in vitro fertilization (IVF) and its application is less widespread. In this paper, we discuss the present status, applications and factors affecting ICSI in pigs and other species. |
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