| 含CpG序列PRRSV ORF5重组质粒在小鼠细胞中的免疫 |
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| 引用本文： | 温建新,王金宝.含CpG序列PRRSV ORF5重组质粒在小鼠细胞中的免疫[J].西北农业学报,2009,18(2):1-5. |
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| 作者姓名： | 温建新 王金宝 |
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| 作者单位： | 1. 山东农业大学,动物科技学院,山东泰安,271018;青岛农业大学,动物科技学院,山东青岛,266109
2. 山东省农业科学院,山东济南,250000 |
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| 摘 要： | 为了提高猪繁殖与呼吸综合征病毒(PRRSV)基因免疫的效果,将成功构建含CpG序列PRRSV ORF5的真核重组表达质粒CpG-pVAX1- ORF5免疫SPF小鼠,检测小鼠的脾T淋巴细胞亚群数量(CD4+和CD8+)以及淋巴细胞增殖反应(MTT法)水平.结果表明,构建的含CpG序列真核重组表达质粒CpG-pVAX1- ORF5能诱导小鼠产生针对PRRSV的细胞免疫.
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF5基因 细胞免疫应答 |
| 收稿时间： | 2008/3/14 0:00:00 |
| 修稿时间： | 2008/5/20 0:00:00 |
Cellular Immune Response Evicited Eukaryotic Expression Vector Containing PRRSV ORF5 and CpG Motifs |
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| WEN Jianxin and WANG Jinbao.Cellular Immune Response Evicited Eukaryotic Expression Vector Containing PRRSV ORF5 and CpG Motifs[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(2):1-5. |
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| Authors: | WEN Jianxin and WANG Jinbao |
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| Institution: | College of Animal Science and Technology, Shandong Agriculture University, Taian Shandong 265200, China;Qindao Agriculture University, Qingdao Shandong 266109, China;Agriculture Research Institute SAAS, Jinan Shandong 250000, China |
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| Abstract: | To improve the effect of the gene immunization against porcine reproductive and respiratory syndrome virus(PRRSV) we constructed the eukaryotic expression vector CpGpVAXl-ORF5 containing PRRSV ORF5 gene coding region and CpG motif.The recombinant expression plasmid was inoculated into SPF mice.The number of CD4+,CD8+ and the lymphocyte proliferation test(MTT) in peripheral blood of mice vaccinated were used to detect.The results showed that the recombinant plasmid CpGpVAXl-ORF5 could induce higher response of cellular immune response. |
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| Keywords: | Porcine reproductive and respiratory syndrome virus (PRRSV) ORFS gene Cellular immune response |
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