| 绿竹cab-BO2基因的克隆及其表达载体的构建 |
| |
| 引用本文： | 高志民,李雪平,岳永德,彭镇华.绿竹cab-BO2基因的克隆及其表达载体的构建[J].分子植物育种,2007,5(3):431-435. |
| |
| 作者姓名： | 高志民 李雪平 岳永德 彭镇华 |
| |
| 作者单位： | 1. 国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京,100102
2. 中国林业科学研究院林业研究所,北京,100091 |
| |
| 基金项目： | 国家林业局引进国际先进农业科学技术计划(948计划) |
| |
| 摘 要： | 捕光叶绿素a/b结合蛋白基因是绿色植物光合系统中的重要基因,其编码的蛋白与色素所形成的蛋白复合体在光化学反应、光保护等方面都起着重要的作用.采用RT-PCR技术和与RACE-PCR技术,从绿竹中克隆到捕光叶绿素a/b结合蛋白基因的全长cDNA编码区,命名为cab-BO2(GenBank登记号:EF088668).该基因编码区长为792 bp,编码263个氨基酸.通过加酶切位点的方法,将基因的编码区直接构建到载体pBI121多克隆位点.经过酶切检测,获得了包含35S启动子、cab基因、GUS报告基因和NOS终止区域的植物表达载体.
|
| 关 键 词： | 绿竹(Dendrocalamopsis oldhami) 捕光叶绿素a/b结合蛋白基因 cab-BO2基因 植物表达载体 |
Cloning and Construction of Expression Vector of cab-BO2 Gene Encoding the Light Harvesting Chlorophyll a/b-binding Protein in Bamboo |
| |
| Gao Zhimin,Li Xueping,Yue Yongde,Peng Zhenhua.Cloning and Construction of Expression Vector of cab-BO2 Gene Encoding the Light Harvesting Chlorophyll a/b-binding Protein in Bamboo[J].Molecular Plant Breeding,2007,5(3):431-435. |
| |
| Authors: | Gao Zhimin Li Xueping Yue Yongde Peng Zhenhua |
| |
| Abstract: | The cab gene coding light harvesting chlorophyll a/b-binding protein is one of key genes in photosyn- thesis system of green plants, which play an important role in light chemical reaction and light protection by bind- ing with Chlorophyll a/b. A full-length cDNA coding region of cab gene was cloned from the first strand of bam- boo cDNA by RT-PCR and RACE methods, which named as cab-BO2 (cab gene 2 from B. oldhamii) (GenBank accession No.: EF088668). The coding region of cab-BO2 is 792 bp and encodes 263 amino acids. The cab-BO2 gene was cloned in the multiple cloning site of pBI121 vector directly by adding restriction enzyme sites. A plant expression vector containing 35S promoter, cab-BO2 gene, GUS and NOS region was confirmed by restriction en- zyme analyses. |
| |
| Keywords: | Bamboo (Bambusa oldhamii) Light harvesting chlorophyll a/b-binding protein gene (cab) cab-BO2 gene Plant expression vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
