Lectin histochemistry of dog major and minor salivary glands |
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| Authors: | Sozmen M Brown P Eveson J |
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| Institution: | Department of Pathology and Microbiology, School of Veterinary Science, University of Bristol Veterinary School, Langford, UK. |
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| Abstract: | The distribution of different carbohydrates in dog major and minor salivary glands was investigated using a peroxidase-labelled avidin-biotin method to demonstrate binding of six lectins (Canavalia ensiformis agglutinin Con A], Dolichos biflorus agglutinin DBA], Arachis hypogaea (peanut) agglutinin PNA], Glycine max agglutinin SBA], Tetragonolobus purpurea agglutinin TGP] and wheat germ agglutinin WGA]). With PNA, there was only weak staining in serous acini of parotid glands. Other lectins bound, to different degrees, to different components of the salivary glands; differences could be detected between glands and between binding of different lectins to serous and mucous acinar cells and to the epithelial cell cytoplasm, luminal surface and contents of ducts. These results provide a basis for the comparison of possible changes in carbohydrates which may occur in salivary gland diseases. |
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