| 眶棘鲈属鱼类DNA条形码及分子系统进化研究 |
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| 引用本文： | 陈厚桦,陈震翰,陈玉佩,叶嘉文,李培源,林蠡,梁日深.眶棘鲈属鱼类DNA条形码及分子系统进化研究[J].仲恺农业工程学院学报,2022,35(2):20-25. |
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| 作者姓名： | 陈厚桦 陈震翰 陈玉佩 叶嘉文 李培源 林蠡 梁日深 |
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| 摘 要： | 为在分子层面分析我国眶棘鲈属（Scolopsis）鱼类系统进化关系，同时探讨COⅠ基因作为DNA条形码在眶棘鲈属鱼类物种鉴定的适用性. 本研究对采集的9种眶棘鲈COⅠ基因部分序列进行PCR扩增及测序，结合GenBank下载的5种眶棘鲈COⅠ基因序列共同分析.结果表明，14种眶棘鲈的种内平均遗传距离为：0.002 5；种间平均遗传距离为0.179 4，种间距离是种内的71.76倍. 在分子系统进化树上，14种眶棘鲈主要形成3个分支：分支Ⅰ包含4种眶棘鲈，这4种眶棘鲈眶下骨均具大棘，与形态学分类结果一致；分支Ⅱ包含4种眶棘鲈，分支Ⅲ包含6种眶棘鲈，但进化树部分节点的支持率相对较低，表明COⅠ基因在眶棘鲈属鱼类中可作为有效的DNA条形码基因以区分物种，但不一定适合用于分子系统进化分析. 另外，部分资料中认为是同种异名的条纹眶棘鲈(Scolopsis taeniopterus)与双斑眶棘鲈（Scolopsis bimaculata）、单带眶棘鲈(Scolopsis monogramma)，彼氏眶棘鲈（Scolopsis affinis）与黄带眶棘鲈(Scolopsis aurata)，本研究COⅠ遗传距离分别为0.160、0.118和0.122，均远大于Hebert推荐的区分不同物种的最小遗传距离2%，各自应分别为独立物种.
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DNA barcoding and molecular phylogeny studies of Scolopsis fish |
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| Abstract: | In order to analyze the molecular phylogenetic relationships of Scolopsis fish in China sea as well as investigate the applicability of COⅠ gene as a DNA barcode for the Scolopsis species identification, partial sequences of COⅠ genes of 9 Scolopsis species were amplified and sequenced, in this study. Combining with 5 COⅠ sequences obtained from GenBank, the molecular phylogenetic relationships of 14 Scolopsis species were constructed and analyzed. The results indicated that: the average intra-species genetic distance values of 14 Scolopsis species was 0.002 5, while the average inter-species genetic distance value was 0.179 4, which was 71.76 times of the former. In the molecular phylogenetic tree, 14 species mainly formed three groups. Group Ⅰ included 4 Scolopsis species, that had big sharp bone below the eyes, which was consistent with morphological classification; Group Ⅱ included 4 species and the Group Ⅲ included 6 species, however the noding support values were relatively low in some branches, indicating that COⅠ gene can be used as an effective DNA barcode gene to distinguish species, but may not suitable for molecular phylogenetic analysis. In addition, the COⅠ genetic distance values of certain species which under morphological synonyms argument (Scolopsis taenioptera+Scolopsis bimaculata,+ Scolopsis monogramma and Scolopsis affinis + Scolopsis aurata) were revealed to be 0.160, 0.118, and 0.122, respectively. The values were all far greator than the minimum distance value (2%) in distinguishing different species recommended by Hebert, suggesting that they should be independent species. |
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