| 果园脐腹小蠹伴生(共生)真菌群落组成及功能分析 |
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| 引用本文： | 朱晓锋,蔡淑琳,苏卓文,张殿朋,宋博,徐兵强,阿布都克尤木 ,卡德尔,杨森.果园脐腹小蠹伴生(共生)真菌群落组成及功能分析[J].新疆农业科学,2022,59(3):691-699. |
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| 作者姓名： | 朱晓锋 蔡淑琳 苏卓文 张殿朋 宋博 徐兵强 阿布都克尤木 卡德尔 杨森 |
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| 作者单位： | 1.农业部西北荒漠绿洲作物有害生物综合治理重点实验室/特色林果产业国家地方联合工程研究中心/新疆农业科学院植物保护研究所,乌鲁木齐 8300912.北京市农林科学院植物保护环境保护研究所,北京 100097 |
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| 基金项目： | 农业部西北荒漠绿洲作物有害生物综合治理重点实验室(KFJJ20180102);新疆杏产业技术体系建设专项 |
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| 摘 要： | 【目的】研究新疆果树脐腹小蠹伴生真菌种类或类群,分析其功能,为脐腹小蠹为害机理和伴生真菌开发利用提供基础信息。【方法】在新疆疏勒、英吉沙等地采集被害果树上的脐腹小蠹成虫,利用MiSeq高通量测序技术和室内分离培养的方法,鉴定其伴生真菌种类或类群,通过FUNGuild功能预测和查阅相关文献,分析其功能。【结果】脐腹小蠹伴生真菌主要由7个门、24个纲、66个目、124个科的221个属组成;体表和肠道伴生真菌群落无显著性差异,体表伴生真菌优势菌群为Geosmithia、Saccharomycetales、Byssochlamys和 Wickerhamomyces,分别占群落的29.55%、19.50%、8.20%和6.07%;而肠道伴生真菌优势菌群为Geosmithia、Pleosporales和 Gibellulopsis,分别占42.14%,15.61%和6.97%;伴生真菌以腐生营养型、植物致病性、病理-腐生营养型和动物致病性为主,且不同果树脐腹小蠹均携带植物病致病性真菌,其中桃树脐腹小蠹携带植物致病型真菌比例最高,为19.06%;室内分离鉴定出伴生真菌13种,其中Geosmithia pallida、Yamadazyma mexicana和Cladosporium macrocarpum为植物病原菌,Wickerhamomyces silvicola、Meyerozyma guilliermondii、Rhodotorula mucilaginosa和Aureobasidium pullulans为多种植物病原菌的拮抗菌。【结论】新疆果树脐腹小蠹普遍携带、传播植物病原真菌,在防控脐腹小蠹的同时应注重果树枝干病害的防控,防止2类有害生物协同为害,加重危害;而伴生真菌中的有益拮抗菌可作为植物病害的生物防治菌被开发利用。
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| 关 键 词： | 果园 脐腹小蠹 伴生真菌 群落结构 功能分析 |
| 收稿时间： | 2021-05-17 |
Study on the Community Structure and Function of Fungi Associated (Symbiotic) with Scolytus schevyrewi That Infest Fruit Trees |
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| ZHU Xiaofeng,CAI Shulin,SU Zhuowen,ZHANG Dianpeng,SONG Bo,XU Bingqiang,Abudukeyoum Kader,YANG Sen.Study on the Community Structure and Function of Fungi Associated (Symbiotic) with Scolytus schevyrewi That Infest Fruit Trees[J].Xinjiang Agricultural Sciences,2022,59(3):691-699. |
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| Authors: | ZHU Xiaofeng CAI Shulin SU Zhuowen ZHANG Dianpeng SONG Bo XU Bingqiang Abudukeyoum Kader YANG Sen |
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| Institution: | 1. Key Laboratory of Intergraded Management of Harmful Crop Vermin of China Northwestern Oasis, MOARA/ National Local Joint Engineering Research Center of Special Forestry and Fruit Industry /Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China2. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China |
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| Abstract: | 【Objective】 To understand the species or group and analysis on the function of fungi associated (symbiotic) with Scolytus schevyrewi in fruit trees in the hope of providing basic information for utilization of these symbionts and revealing the damage mechanism of S.schevyrewi.【Method】 S.schevyrewi adults were collected from infested fruit trees in Shule, Yingjisha and so on, the species and group of fungi associated with S.schevyrewi were identified by using MiSeq high-throughput sequencing technology and indoor isolation and culture methods.The functions of the fungi were analyzed by using FUN Guild and referring to relevant literatures.【Result】 The fungi belonged to 7 Phylum, 24 Class, 66 Order, 124 Family, 221 Genus.The dominant fungal microflora of the surface of S.schevyrewi were Geosmithia, Saccharomycetales, Byssochlamys and Wickerhamomyces, which accounted for 29.55%, 19.50%, 8.20% and 6.07% of the communities, respectively.The dominant fungal microfloras of the gut of S.schevyrewi include Geosmithia, Pleosporales, Gibellulopsis, accounting for 42.14%, 15.61% and 6.97%, respectively.The fungi were mainly saprotroph, plant pathogen, pathotroph- saprotroph and animal pathogen.S.schevyrewi adults, which infested apricot, plum, peach and almond trees, coexisted with a variety of plant-pathogenic fungi.The proportion of plant-pathogenic fungi was the highest in S.schevyrewi adults that infested peach trees, which was 19.06%.Thirteen associated fungi were isolated and identified in laboratory.Among them Geosmithia pallida, Yamadazyma Mexicana and Cladosporium Macrocarpum were plant pathogens.Wickerhamomyces Silvicola, Meyerozyma Guilliermondii, Rhodotorula mucilaginosa and Aureobasidium pullulans were antagonists of the plant-pathogens.【Conclusion】 It was common to carry and spread plant-pathogens by S.schevyrewi in Xinjiang fruit trees.When preventing and controlling S.schevyrewi, attention should be paid to the prevention and control of fruit tree trunk diseases, so as to prevent the two kinds of pests from synergistic damage and aggravating the damage.The beneficial antagonistic fungi associated with S.schevyrewi should be exploited and used as biological control fungi for some plant diseases. |
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| Keywords: | orchard Scolytus schevyrewi Semenov associated fungus community structure function |
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