| 桃XTH家族基因鉴定及其在桃果实贮藏过程中的表达特性 |
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| 引用本文： | 郭绍雷,许建兰,王晓俊,宿子文,张斌斌,马瑞娟,俞明亮.桃XTH家族基因鉴定及其在桃果实贮藏过程中的表达特性[J].中国农业科学,2022,55(23):4702-4716. |
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| 作者姓名： | 郭绍雷 许建兰 王晓俊 宿子文 张斌斌 马瑞娟 俞明亮 |
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| 作者单位： | 1江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，南京 2100142南京农业大学园艺学院，南京 210095 |
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| 基金项目： | 江苏省重大新品种创制(PZCZ201727);现代农业产业技术体系建设专项资金(CARS-30) |
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| 摘 要： |
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| 关 键 词： | 桃 果实软化 XTH基因家族 基因表达 亚细胞定位 |
| 收稿时间： | 2022-02-23 |
Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage |
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| GUO ShaoLei,XU JianLan,WANG XiaoJun,SU ZiWen,ZHANG BinBin,MA RuiJuan,YU MingLiang.Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage[J].Scientia Agricultura Sinica,2022,55(23):4702-4716. |
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| Authors: | GUO ShaoLei XU JianLan WANG XiaoJun SU ZiWen ZHANG BinBin MA RuiJuan YU MingLiang |
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| Institution: | 1Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 2100142College of Horticulture, Nanjing Agricultural University, Nanjing 210095 |
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| Abstract: | 【Objective】 The aim of this study was to identify members of the XTH gene family from peach, and to analyze the expression of PpXTHs in peach fruit with different textures during storage, which not only provided data for the research on the candidate PpXTHs involved in peach fruit softening, but also laid the foundation for further study on the PpXTHs function in peach fruit softening.【Method】 The HMM profiles of the Glyco_hydro_16 domain and XET_C domain were used to search all XTH proteins with the Hmmer 3.1 software in the peach protein database. The molecular weight, theoretical isoelectric point and other physicochemical properties were then predicted by the online tool ProtParam. PpXTHs subcellular localization were predicted by the online software Plant-mPLoc. The MEGA 11 software was used to construct a phylogenetic tree. The online tool MEME was used to analyze conserved motifs, the conserved motifs, conserved protein domains and gene structure maps were draw by Tbtools. According to the PpXTH gene family location information, chromosome mapping was carried out with MapChart software. The expressions of PpXTHs in peach fruit with different textures during storage were monitored by qRT-PCR..【Result】 A total of 27 PpXTH genes were systematically identified from peach, which were distributed on seven chromosomes. Based on the phylogenetic tree, the ancestral group, Ⅰ/Ⅱ subfamily, ⅢA subfamily and ⅢB subfamily were classified. In addition, according to the analysis of protein domains, all PpXTH gene family proteins had Glyco_hydro_16 and XET_C conserved domain. The results from qRT-PCR analysis showed that PpXTH33 belonging to the ⅢB subfamily was upregulated as the storage period increased in melting peach fruit, with the expression being markedly higher than that during the storage period of stony hard peach fruit. The positive clone sequencing was consistent with the coding sequence of the Prunus persica reference genome with a length of 924 bp for a 307 amino acid sequence. The PpXTH33 combined with green fluorescent protein may mainly located in the cell wall and nucleus detected by confocal laser scanning microscopy..【Conclusion】 All 27 PpXTHs protein structures contained two highly XTH conserved domains and the genes were distributed on seven chromosomes. The expression characteristics of PpXTH33 in peach fruit with different flesh textures during storage suggested that PpXTH33 was closely associated with peach fruit softening during storage. |
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| Keywords: | peach fruit softening XTH gene family gene expression subcellular localization |
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