| 尿素对蛋白激发子AMEP功能活性的影响 |
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| 引用本文： | 万恒东,岳文涛,赵锋,商宪富,陈道文,王思文,孙明超,刘权.尿素对蛋白激发子AMEP功能活性的影响[J].现代农业科技,2022(2). |
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| 作者姓名： | 万恒东 岳文涛 赵锋 商宪富 陈道文 王思文 孙明超 刘权 |
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| 作者单位： | 黑龙江八一农垦大学,北大荒集团九三分公司红五月农场,北大荒集团九三分公司红五月农场,北大荒集团九三分公司红五月农场,北大荒集团九三分公司红五月农场,黑龙江八一农垦大学,黑龙江八一农垦大学,黑龙江八一农垦大学 |
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| 基金项目： | 2021年“全国基层农技推广体系改革与建设补助项目(AMEP蛋白免疫激活剂在九三大豆的绿色增产示范),黑龙江省八一农垦大学试验示范基地项目(AMEP蛋白免疫激活剂在九三大豆的应用) |
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| 摘 要： | AMEP是从枯草芽孢杆菌BU412中提取出的一种新型的蛋白激发子,该蛋白激发子可以提高植物的抗病性,是理想的生物农药的候选材料。AMEP在施用过程中与叶面肥混用是目前药肥一体化研究的热点。尿素是叶面肥的主要组成,同时也是蛋白质的变性剂。高浓度的尿素可以改变蛋白质的结构,使蛋白质失去原有性质,其对AMEP蛋白的功能活性是否产生影响还有待阐明。本文为了探究尿素对蛋白激发子AMEP功能活性的影响,设置了一系列浓度梯度的尿素对AMEP蛋白进行处理,通过烟草叶片的过敏反应来检测尿素对AMEP的功能活性影响。结果表明,低浓度的尿素不会影响AMEP的功能活性；而尿素浓度超过0.5mg/mL,AMEP的功能活性则受到明显抑制。随后,本文通过灰霉菌发病实验确认了0.05mg/mL尿素处理的AMEP能够提高植物的抗病性。本研究确定AMEP可与0.05mg/mL尿素配伍施用,为尿素与AMEP的共同施用提供了理论依据。
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| 关 键 词： | AMEP 尿素 枯草芽孢杆菌 叶面肥 灰霉菌 |
| 收稿时间： | 2021/5/19 0:00:00 |
| 修稿时间： | 2021/5/19 0:00:00 |
Effect of urea on the functional activity of protein elicitor AMEP |
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| Authors: | LIU Quan |
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| Institution: | Heilongjiang Bayi Agricultural University,Daqing |
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| Abstract: | AMEP is a new Protein Elicitor extracted from Bacillus subtilis bu412, which can improve the disease resistance of plants and is an ideal candidate for biological pesticides. The application of AMEP in combination with foliar fertilizer is a hot spot in the research of the integration of medicine and fertilizer. Urea is not only the main component of foliar fertilizer, but also the denaturant of protein. High concentration of urea can change the structure of protein and make the protein lose its original properties. Whether it affects the functional activity of AMEP remains to be clarified. In order to explore the effect of urea on the functional activity of Protein Elicitor AMEP, a series of concentration gradient urea was set to treat AMEP protein, and the effect of urea on the functional activity of AMEP was detected by the allergic reaction of tobacco leaves. The results showed that low concentration of urea did not affect the functional activity of AMEP; When the concentration of urea was more than 0.5mg/ml, the functional activity of AMEP was significantly inhibited. Subsequently, we confirmed that AMEP treated with 0.05mg/ml urea could improve the disease resistance of plants through the experiment of gray mold. This study determined that AMEP could be applied with 0.05mg/ml urea, which provided a theoretical basis for the joint application of urea and AMEP. |
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| Keywords: | AMEP Urea Bacillus subtilis Foliar fertilizer Grey mould |
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