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Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis |
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| Authors: | Yuta KINOSHITA Hironaga KAKOI Taichiro ISHIGE Takashi YAMANAKA Hidekazu NIWA Eri UCHIDA-FUJII Toshio NUKADA Takanori UENO |
| Institution: | 1)Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan;2)Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan;3)Epizootic Prevention Section, Equine Department, Japan Racing Association, 1-1-1 Nishi Shinnbashi, Minato-ku, Tokyo 105-0003, Japan |
| Abstract: | Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples. |
| Keywords: | contagious equine metritis nucleic acid amplification test real-time PCR Taylorella equigenitalis |