| 芽胞杆菌JZB30-1的鉴定及其对番茄灰霉病的生防作用 |
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| 引用本文： | 赵 娟,左文杰,张殿朋,秦文韬,卢彩鸽.芽胞杆菌JZB30-1的鉴定及其对番茄灰霉病的生防作用[J].植物保护,2024,50(3):165-175. |
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| 作者姓名： | 赵 娟 左文杰 张殿朋 秦文韬 卢彩鸽 |
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| 作者单位： | 1. 北京市农林科学院植物保护研究所, 北京 100097; 2. 农业农村部北方果蔬有害生物绿色防控重点实验室(部省共建), 北京 100097; 3. 北京农学院生物与资源环境学院, 北京 102206 |
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| 基金项目： | 北京市农林科学院创新能力建设专项（KJCX20210402） |
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| 摘 要： | 为获得对番茄灰霉病具有良好防效的生防芽胞杆菌, 采用对峙培养、双层平板以及生防相关特性检测等方法筛选高活性拮抗菌株, 根据形态学、生理生化特性及多基因测序进行菌株种类鉴定, 通过离体果实和盆栽试验明确供试菌株的防病促生效果。结果表明, 芽胞杆菌JZB30-1对番茄灰霉病菌等10余种植物病原真菌和细菌具有广谱抑菌活性; 该菌株具有合成蛋白酶、纤维素酶、脂肽类物质，分泌铁载体、生长激素及溶磷等特性, 经鉴定其为解淀粉芽胞杆菌Bacillus amyloliquefaciens; 菌株JZB30-1无菌发酵滤液50倍液喷施处理, 对番茄果实灰霉病防效为95.0%, 发酵液10倍稀释液灌根, 番茄株高、鲜重分别较对照增加25.7%和28.4%。相关结果为利用解淀粉芽胞杆菌JZB30-1进行番茄灰霉病生物防治提供理论依据。
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| 关 键 词： | 番茄灰霉病 解淀粉芽胞杆菌 抑菌活性 功能基因 生物防治 |
| 收稿时间： | 2023/4/7 0:00:00 |
| 修稿时间： | 2023/9/28 0:00:00 |
Identification of Bacillus strain JZB30-1 and its biological control effect on tomato grey mould |
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| ZHAO Juan,ZUO Wenjie,ZHANG Dianpeng,QIN Wentao,LU Caige.Identification of Bacillus strain JZB30-1 and its biological control effect on tomato grey mould[J].Plant Protection,2024,50(3):165-175. |
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| Authors: | ZHAO Juan ZUO Wenjie ZHANG Dianpeng QIN Wentao LU Caige |
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| Institution: | 1. Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; 2. Key Laboratory of Environment-Friendly Management on Fruit and Vegetable Pests in North China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Beijing 100097, China; 3. College of Bioscience, Resources and Environment, Beijing University of Agriculture, Beijing 102206, China |
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| Abstract: | To obtain a Bacillus strain with significant control effect on tomato grey mould, antagonistic bacteria were screened by confrontation culture and two-layer plate methods, along with the detection of characteristics related to biocontrol. Its taxonomic status was identified based on morphological features, physiological-biochemical characteristics, and multiple gene sequences analyses. The disease-prevention and growth promotion effects of the obtained strain were evaluated by applying the fermentation filtrate of the strain to tomato fruits and potted tomato seedlings, respectively. The results showed that Bacillus strain JZB30-1 exhibited an obvious inhibitory effect on Botrytis cinerea, as well as broad-spectrum antifungal and antibacterial activities against more than ten plant pathogens. The strain was identified as Bacillus amyloliquefaciens, which have features of synthesizing protease, cellulase and lipopeptide, secreting siderophore, indoleacetic acid and solubilizing phosphate. The 50-fold sterile fermention filtrate of JZB30-1 displayed an obvious biocontrol effect on tomato fruit grey mould, with a 95.0% reduction in disease incidence compared to the control. In addition, the plant height and fresh weight increased by 25.7% and 28.4%, respectively, after root-irrigation with a 10-fold dilution of the fermentation. The study provides a theoretical foundation for considering B. amyloliquefaciens strain JZB30-1 as a potential biocontrol strain against tomato grey mould. |
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| Keywords: | tomato grey mould Bacillus amyloliquefaciens antagonistic activity functional genes biological control |
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