| 百合‘白天使’与山丹远缘杂交胚胎发育的细胞学研究 |
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| 引用本文： | 李小英,王文和,赵剑颖,王树栋,赵祥云.百合‘白天使’与山丹远缘杂交胚胎发育的细胞学研究[J].园艺学报,2010,37(2):256-261. |
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| 作者姓名： | 李小英 王文和 赵剑颖 王树栋 赵祥云 |
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| 作者单位： | 北京农学院园林系,北京,102206 |
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| 基金项目： | 北京市教委基金项目，北京市园林绿化局项目，北京市属市管高等学校人才强教计划项目 |
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| 摘 要： | 采用石蜡切片法对以亚洲百合品种‘白天使’为母本,野生种山丹为父本杂交后不同发育时期的胚珠进行制片观察,发现白天使×山丹远缘杂交受精和胚胎发育过程比较复杂,共有4种情况,即:不能完成受精,胚囊成员细胞最后解体;卵受精,只产生胚而无胚乳;极核受精,只产生胚乳而无胚;完成双受精,产生了胚和胚乳。百合杂种胚发育经过合子、原胚、球形胚、偏梨形胚、棒状胚阶段。偏梨形胚之前,胚的发育所需要的营养主要是通过胚柄从珠心和珠被中吸收。当胚体内部开始分化,外形呈棒状,胚柄解体。此后胚发育所需的营养则主要由胚乳细胞提供。百合胚乳发育为核型。杂种胚败育的可能原因是胚没有得到胚乳的哺育,这样的胚最多发育到球形胚阶段;其次是胚乳发育缓慢或停止,最后导致胚的夭折。该杂交组合授粉后45d,是进行胚抢救的最佳时期。
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| 关 键 词： | 百合 远缘杂交 胚胎发育 |
| 收稿时间： | 2009-11-11 |
| 修稿时间： | 2010-1-22 |
Cytological Studies on Distant Hybridization Embryonic Development of Lilium |
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| LI Xiao-ying,WANG Wen-he,ZHAO Jian-ying,WANG Shu-dong, ZHAO Xiang-yun.Cytological Studies on Distant Hybridization Embryonic Development of Lilium[J].Acta Horticulturae Sinica,2010,37(2):256-261. |
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| Authors: | LI Xiao-ying WANG Wen-he ZHAO Jian-ying WANG Shu-dong ZHAO Xiang-yun |
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| Institution: | 1.Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China;2.Dongguan Peoples Hospital, Dongguan 511700, China. E-mail: dengyub@mail.sysu.edu.cn |
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| Abstract: | This experiment was based on paraffin methods to observe ovule of different development stages in hybrid combination between Lilium asiatic hybrids‘Navona’× Lilium pumilum,while the female parent was Asiatic hybrids and male parent is wild type.We found that fertilization and embryonic development process of this distant hybrid combination were complicated,there were four conditions during the development.Fertilization could not be completed and embryonic sac member cells would disintegrate at last,which was one of the conditions.The second one was only the egg cell be fertilized,embryo will be obtained without endosperm.The third one was just the opposite,only polar nuclear cells were fertilized and endosperm be obtained but embryo.The last condition was double fertilization completed,both embryo and endosperm were obtained.Development of hybrid idiosome in Lilium went through stages of zygote,proembryo,spherical embryo,pear-shaped like embryo and clavate embryo.Before the clavate embryo stage,nutrition which supporting the embryo development was mainly absorbed from nucellus and integument bythe suspensor.When the embryo began to differentiate within the body,its shape was rod-like,suspensor disintegration.After that,embryo's nutritional requirements were mainly provided by the endosperm cells.Development of Lilium endosperm was belonged to nuclear type.One of the reasons which probably inducing hybrid embryonic abortion was no nurture do embryo gain from endosperm,and this kind of embryo would grow not better than the stage of spherical embryo,another probable reason was endosperm growing slowly or even stop growing,which resulting in embryonic abortion at last.Forty-five days after pollination was the best time to carry out embryo rescue in this hybrid combination. |
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| Keywords: | Lilium distant hybridization embryonic development |
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