The Induction of Tetraploidy in Salmonids期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

按检索

The Induction of Tetraploidy in Salmonids

Authors:	James M Myers William K Hershberger Robert N Iwamoto

Institution:	School of Fisheries, University of Washington, Seattle, Washington 98195 USA;Domsea Farms, Inc., 4398 W. Old. Belfair Hwy., Bremerton, Washington 98312 USA

Abstract:	The induction of tetraploidy was attempted in three intraspecies crosses (autotetraploid) of sal-monid: chinook salmon ( Oncorhynchus tshawytscha ), coho salmon ( O. kisutch ) and rainbow trout ( Salmo gairdneri ), and in the interspecies hybrid (allotetraploid) coho × Atlantic salmon ( Salmo salar ). Pressure treatments of 6.2 × 10⁴ kPa (9,000 psi) were applied for six minutes at intervals from 50% of the time to cleavage to beyond first cleavage. Analysis of eyed stage embryos via flow cytometry indicated two periods of tetraploid induction existed corresponding to 55–75% and 100–110% of the first cleavage interval (FCI). The effects of different pressures and the times of application were analyzed in a study with rainbow trout. Treatments of 4.8 × 10⁴ kPa (7,000 psi) for eight minutes, and 5.5 and 6.2 × 10⁴ kPa (8,000 and 9,000 psi) for four minutes, were applied at 65, 78, and 100% FCI. Tetraploids were found in the 5.5 and 6.2 × 10⁴ kPa group treatments only when applied at 65% FCI. The results indicate that, while tetraploidy is inducible in salmonids, further experimentation is necessary to discern the appropriate time and treatment for optimal tetraploid induction in each species.

Keywords:

设为首页 | 免责声明 | 关于勤云 | 加入收藏