| 钠、钾离子对Bt蛋白在蒙脱石和凹凸棒土表面吸附与解吸的影响 |
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| 引用本文： | 刘宁,周学永,董庆洁,刘祥云,姚奇才,黄晓林.钠、钾离子对Bt蛋白在蒙脱石和凹凸棒土表面吸附与解吸的影响[J].农业环境保护,2012(4):786-790. |
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| 作者姓名： | 刘宁 周学永 董庆洁 刘祥云 姚奇才 黄晓林 |
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| 作者单位： | [1]天津理工大学化学化工学院,天津300384 [2]天津农学院食品科学系,天津300384 |
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| 基金项目： | 国家自然科学基金项目（31070478）; 天津市自然科学基金重点项目（08JCZDJC18800）; 天津农学院科技发展计划（2008D016） |
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| 摘 要： | 采用静态吸附法研究了钠、钾离子对苏云金杆菌杀虫蛋白在蒙脱石和凹凸棒土表面吸附与解吸特性的影响。结果表明,添加钠、钾离子（0~2 mmol.L-1）均可提高Bt蛋白在两种矿物上的平衡吸附量,但等温吸附曲线的Langmuir方程拟合基本没受影响。添加钠离子或钾离子均能引起矿物粒子Zeta负电位升高,提示钠、钾离子通过减小矿物粒子与Bt蛋白之间斥力的方式促进了Bt蛋白吸附。在蒙脱石吸附Bt蛋白过程中添加钠、钾离子可使Bt蛋白的水解吸率升高,但解吸后的残留量变化不大;在凹凸棒土吸附Bt蛋白过程中添加钠、钾离子可使Bt蛋白的水解吸率减小,且解吸后的残留量增加。
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| 关 键 词： | Bt蛋白 蒙脱石 凹凸棒土 钠、钾离子 吸附与解吸 |
Effects of Sodium and Potassium Ion on the Adsorption and Desorption of Bt Protein by Montmorillonite and Attapulgite |
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| LIU Ning,ZHOU Xue-yong,DONG Qing-jie,LIU Xiang-yun,YAO Qi-cai,HUANG Xiao-lin.Effects of Sodium and Potassium Ion on the Adsorption and Desorption of Bt Protein by Montmorillonite and Attapulgite[J].Agro-Environmental Protection,2012(4):786-790. |
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| Authors: | LIU Ning ZHOU Xue-yong DONG Qing-jie LIU Xiang-yun YAO Qi-cai HUANG Xiao-lin |
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| Institution: | 1.College of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300384,China;2.Department of Food Science,Tianjin Agricultural University,Tianjin 300384,China) |
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| Abstract: | The effects of sodium and potassium ion on the adsorption and desorption characteristics of the insecticidal protein from Bacillus thuringiensis（Bt） by montmorillonite and attapulgite were studied by a batch method.The results showed that the adsorption amount of Bt protein by both minerals increased with the addition of sodium or potassium ion（0~2 mmol·L-1）.The fitting of isotherms for Langmuir equation,however,was not influenced.The Zeta negative potential of mineral suspensions increased by the addition of sodium or potassium ion,suggesting that the adsorption of Bt protein on minerals was promoted by sodium or potassium ion through the reduction of repulsion between mineral particles and Bt protein.The addition of sodium or potassium ion during the adsorption of Bt protein by montmorillonite resulted in a increase of desorption of Bt protein,but the residual quantity of Bt protein had a slight change.The addition of sodium or potassium ion during the adsorption of Bt protein by attapulgite,however,resulted in a decrease of desorption of Bt protein,and the residual quantity of Bt protein increased. |
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| Keywords: | Bt protein montmorillonite attapulgite sodium and potassium ion adsorption and desorption |
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