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| 不同药物对动脉粥样硬化兔体内两种受体表达的影响 | |
| 引用本文： | 崔亚利,郑辉,胡满,任旭兵,赵德明.不同药物对动脉粥样硬化兔体内两种受体表达的影响[J].河北农业大学学报,2006,29(5):98-102. |
| 作者姓名： | 崔亚利 郑辉 胡满 任旭兵 赵德明 |
| 作者单位： | 1. 河北农业大学,动物科技学院,河北,保定,071001 2. 河北农业大学,教务处,河北,保定,071001 3. 中国农业大学,动物医学院,北京,海淀,100094 |
| 基金项目： | 教育部博士点基金项目(20020019006) |
| 摘 要： | 为了给药物的筛选和利用提供基础数据,用手术法切除新西兰雌兔双侧卵巢,用高胆固醇饮食法制备动脉粥样硬化模型。利用实时荧光定量RT-PCR方法,对动脉粥样硬化兔心脏和肝脏内ER mRNA和LDLRmRNA的表达进行了分析。研究表明,ERα在模型组(C)、假手术组(SC)、正常饲料组(N)、利维爱组(TIB)、倍美力组(PRE)和新伐他汀组(SIM)心脏内的相对表达量分别为0.29、0.53、0.46、0.85、0.48、0.30,在肝脏内的相对表达量分别为0.32、0.51、0.49、0.68、0.50、0.30;低密度脂蛋白受体(LDLR)在模型组(C)、假手术组(SC)、正常饲料组(N)、利维爱组(TIB)、倍美力组(PRE)和新伐他汀组(SIM)心脏内的相对表达量分别为0.22、0.24、0.33、0.27、0.31、0.23,在肝脏内的表达量分别为0.86、0.93、1.52、1.27、1.19、0.88。结果显示,利维爱通过增加心脏和肝脏内雌激素受体、低密度脂蛋白受体的表达发挥抗动脉粥样硬化的作用。新伐他汀可以有效的防治动脉粥样硬化的发展,对心脏和肝脏内的2种受体的表达没有影响。倍美力防治AS的效果不明显,可以促进ERα和LDLR在心脏和肝脏内的表达。 |
| 关 键 词： | 动脉粥样硬化 兔 实时荧光定量RT-PCR 受体 利维爱 倍美力 新伐他汀 |
| 文章编号： | 1000-1573（2006）05-0098-05 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-03-10 |
Effect of different medicament on the receptor expression in the atherosclerotic rabbits |
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| CUI Ya-li,ZHENG Hui,HU Man,REN Xu-bing,ZHAO De-ming.Effect of different medicament on the receptor expression in the atherosclerotic rabbits[J].Journal of Agricultural University of Hebei,2006,29(5):98-102. | |
| Authors: | CUI Ya-li ZHENG Hui HU Man REN Xu-bing ZHAO De-ming |
| Institution: | 1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 2. Office of Education Administrator, Agricultural University of Hebei, Baoding 071001, China; 3. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China |
| Abstract: | Atherosclerotic animal model with oophorectomized rabbits and high cholesterol feedstuff was made.With real-time fluorescence quantitive RT-PCR,we studied the expression of estrogen receptor α and low density lipoprotein receptor mRNA in heart and liver from atherosclerotic rabbits.The results showed: the relative expression of ERα in heart from model group(C),sham-operation group(SC),standard chow group(N),tibolone group(TIB),premarine group(PRE),Simvastatin group(SIM) was 0.29,0.53,0.46,0.85,0.48,0.30 respectively,and was 0.32,0.51,0.49,0.68,0.50,0.30 respectively in liver;the relative expression of LDLR in heart from group C,group SC,group N,group TIB,group PRE,group SIM was 0.22,0.24,0.33,0.27,0.31,0.23 respectively,and was 0.86,0.93,1.52,1.27,1.19,0.88 respectively in liver.Livial has the function of anti-atherosclerosis by stimulating the expression of ERα and LDLR mRNA.Simvastatin can reduce the progression of AS,whereas has no effect on the two receptors.Premarine increased the level of ERα and LDLR mRNA in heart and liver,but has no significant effect on AS plaque. |
| Keywords: | atherosclerosis rabbit real-time RT-PCR receptor livial premarine simvastatin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |