| 蜡样芽胞杆菌905铜锌超氧化物歧化酶基因的克隆及原核表达 |
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| 引用本文： | 莫小丹,王勇军,王琦,梅汝鸿.蜡样芽胞杆菌905铜锌超氧化物歧化酶基因的克隆及原核表达[J].农业生物技术学报,2008,16(3). |
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| 作者姓名： | 莫小丹 王勇军 王琦 梅汝鸿 |
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| 作者单位： | 中国农业大学植物病理学系,北京,100094 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 蜡样芽孢杆菌905（Bacillus cereus 905）是从小麦体内分离获得的一株植物有益内生细菌。为从氧自由基毒性角度分析该细菌在植物体内的定殖机制，本研究利用PCR方法得到了B. cereus 905的CuZn-SOD基因全长（sodC）。该基因由537 bp组成，编码179个氨基酸残基。构建表达载体pET-22b-sodC，转化Escherichia coli BL21(DE3)，IPTG诱导表达结果表明：该基因表达出的蛋白表现出SOD功能，化学抑制法验证其为CuZn-SOD。
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| 关 键 词： | 蜡样芽胞杆菌 铜锌超氧化物歧化酶 原核表达 |
| 收稿时间： | 2007-10-11 |
| 修稿时间： | 2008-4-8 |
Clone of CuZn-superoxide Dismutase(sodC) Gene from Bacillus cereus 905 and Its Expression in Escherichia coli |
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| MO Xiao-dan,WANG Yong-jun,WANG Qi,Mei Ru-hong.Clone of CuZn-superoxide Dismutase(sodC) Gene from Bacillus cereus 905 and Its Expression in Escherichia coli[J].Journal of Agricultural Biotechnology,2008,16(3). |
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| Authors: | MO Xiao-dan WANG Yong-jun WANG Qi Mei Ru-hong |
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| Abstract: | Bacillus cereus strain 905 isolated from wheat is an endophytic beneficial bacterium that can promote plant growth. In this study, the gene encoding CuZn-SOD in B. cereus 905 was cloned by using of PCR to analyze the toxicity of active oxygen species (AOS) to the bacteria during the colonization in plant. The sequence analysis shows that this gene contains 537 base pairs which encoding 179 amino acid residues. For studying the encoded peptide characteristics, the expression vector pET-sodC in Escherichia coli BL21(DE3) was constructed. Results showed the induced protein with IPTG was confirmed as a superoxide dismutase by dyed with NBT. Finally, the gene in B. cereus 905 encoding CuZn-SOD was identified on the basis of inhibited SOD activity by potassium cyanide (KCN) and hydrogen peroxide (H2O2). |
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