| 大豆抗孢囊线虫3号生理小种相关基因PCR检测方法的建立与应用 |
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| 引用本文： | 陈观水,周以飞,潘大仁.大豆抗孢囊线虫3号生理小种相关基因PCR检测方法的建立与应用[J].福建农林大学学报(自然科学版),2004,33(3):359-362. |
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| 作者姓名： | 陈观水 周以飞 潘大仁 |
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| 作者单位： | 福建农林大学作物科学学院,福建,福州,350002 |
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| 基金项目： | 福建省自然科学基金资助项目(B0210017). |
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| 摘 要： | 根据甜菜的抗线虫病基因保守序列,设计合成了4对引物,从中筛选出1对引物,对8份经抗孢囊线虫3号生理小种常规鉴定的大豆品种(6份抗病、2份感病)进行了PCR检测.结果表明,6份抗病品种出现了1条600bp的特异片段,而2份感病品种未出现任何条带.这与常规鉴定结果相一致.PCR Sothern杂交验证了所获得特异片段与抗线虫病基因的同源性,证明使用该方法检测大豆抗孢囊线虫病基因简单、快速、方便、可行.
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| 关 键 词： | 大豆 孢囊线虫病 PCR检测 |
| 文章编号： | 1006-7817(2004)03-0359-04 |
| 修稿时间： | 2004年3月4日 |
Design and application of a PCR-based assay for resistant genes to race 3 of soybean cyst nematode in soybean |
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| CHEN Guan-shui,ZHOU Yi-fei,PAN Da-ren.Design and application of a PCR-based assay for resistant genes to race 3 of soybean cyst nematode in soybean[J].Journal of Fujian Agricultural and Forestry University,2004,33(3):359-362. |
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| Authors: | CHEN Guan-shui ZHOU Yi-fei PAN Da-ren |
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| Abstract: | Based on the sequence of cyst nematode resistant gene in sugar beet, 4 pairs of primers were designed. A pair of primers were selected among them. Eight soybean cultivars (including 6 nematode resistant varieties to SCN race 3, 2 susceptible varieties to SCN race 3 by conventional identification) were screened by using the pair of primers established for cyst nematode resistant gene in soybean. The result was in correspondence with that of conventional identification. PCR-Southern blot proved that it is a feasible, simple, rapid and applicable method for cyst nematode resistant gene in soybean. |
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| Keywords: | soybean cyst nematode disease PCR detection |
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