| 鼠伤寒沙门菌sapC基因缺失株的构建及生物学特性分析 |
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| 引用本文： | 曹莉,程如楠,武周慧,王家伟,王瑜,张永红,吴清民,王真.鼠伤寒沙门菌sapC基因缺失株的构建及生物学特性分析[J].畜牧兽医学报,2022,53(7):2282-2289. |
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| 作者姓名： | 曹莉 程如楠 武周慧 王家伟 王瑜 张永红 吴清民 王真 |
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| 作者单位： | 1. 北京农学院动物科学技术学院, 兽医学(中兽医)北京市重点实验室, 北京 102206;2. 中国农业大学动物医学院, 北京 100193;3. 北京大学第三医院实验动物中心, 北京 100191 |
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| 基金项目： | 北京市优秀人才培养资助项目(2017000026833ZK18)； |
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| 摘 要： | 旨在研究鼠伤寒沙门菌ABC转运膜蛋白SapC在沙门菌致病机制中的功能。本研究利用λ-Red重组技术构建了鼠伤寒沙门菌sapC基因缺失突变株SMΔsapC，对其进行生长特性、酸性应激试验、多黏菌素B敏感性试验、生物被膜检测、胞内存活和小鼠体内毒力试验。结果显示，基因缺失株SMΔsapC与亲本菌株和互补菌株相比，其生长速度无明显差异；在酸应激条件下，SMΔsapC存活率显著低于亲本菌株；sapC基因缺失降低了鼠伤寒沙门菌生物被膜的形成能力；同时，SMΔsapC基因缺失株在鼠源巨噬细胞内的增殖能力和小鼠体内的毒力显著低于亲本菌株。研究表明，sapC基因影响鼠伤寒沙门菌的抗酸能力、生物被膜形成能力，从而影响沙门菌在体内外的毒力。本研究为进一步阐释鼠伤寒沙门菌的致病机制奠定了基础。
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| 关 键 词： | 鼠伤寒沙门菌 同源重组 sapC 生物学特性 |
| 收稿时间： | 2021-10-26 |
Construction and Biological Characteristics of sapC Gene Deletion Strain of Salmonella Typhimurium |
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| CAO Li,CHENG Runan,WU Zhouhui,WANG Jiawei,WANG Yu,ZHANG Yonghong,WU Qingmin,WANG Zhen.Construction and Biological Characteristics of sapC Gene Deletion Strain of Salmonella Typhimurium[J].Acta Veterinaria et Zootechnica Sinica,2022,53(7):2282-2289. |
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| Authors: | CAO Li CHENG Runan WU Zhouhui WANG Jiawei WANG Yu ZHANG Yonghong WU Qingmin WANG Zhen |
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| Institution: | 1. Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China;2. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;3. Animal Experiment Center, Peking University Third Hospital, Beijing 100191, China |
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| Abstract: | This study aimed to study the function of sapC in ABC transporters of SalmonellaTyphimurium in its pathogenic mechanism. The sapC gene deletion mutant SM△sapC of S. Typhimurium was constructed by λ-Red mediated mutagenesis procedures, and the growth characteristics, acid stress test, polymyxin B sensitivity test, biofilm detection, intracellular survival and toxicity test in mice were performed. The results showed that the growth rate of the deletion strain SMΔsapC was not significantly different from that of the parent strain and the supplemented strain. Under acid stress condition, the survival rate of SMΔsapCsapC was significantly lower than that of the parent strain; the deletion of sapC gene enhanced the drug resistance of polymyxin B and decreased the ability of biofilm formation of S. Typhimurium. The proliferation ability of SMΔsapC strain in mouse macrophages and the virulence in mice were significantly lower than that of the parent strain. This study showed sapC gene decreased the acid resistance and biofilm formation ability of S. Typhimurium, thus reducing the virulence of Salmonella in vivo and in vitro. This study laid a foundation for further explaining the pathogenic mechanism of S.Typhimurium. |
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| Keywords: | Salmonella Typhimurium homologous recombination sapC biological characteristics |
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