| 2017-2021年成都市猫细小病毒的检测及其VP2基因的变异分析 |
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| 引用本文： | 周群,杨苑,宋鑫,何欣怡,曹慧,张斌.2017-2021年成都市猫细小病毒的检测及其VP2基因的变异分析[J].畜牧兽医学报,2022,53(4):1303-1309. |
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| 作者姓名： | 周群 杨苑 宋鑫 何欣怡 曹慧 张斌 |
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| 作者单位： | 1. 西南民族大学畜牧兽医学院, 成都 610041;2. 青藏高原动物遗传资源保护 与利用教育部/四川省重点实验室, 成都 610041 |
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| 基金项目： | 四川应用基础研究计划(2020YJ0247)； |
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| 摘 要： | 本研究旨在通过对成都地区家养猫细小病毒(feline parvovirus,FPV)的分子检测,了解FPV在成都地区的流行及遗传变异情况.2017-2021年分别从四川成都地区采集168份家养猫的腹泻粪便样本,用PCR方法对168份样本进行FPV检测,并选取13份阳性样本进行VP2基因全长的克隆和测序,用Meg Ali...
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| 关 键 词： | 猫细小病毒 分子流行病学 VP2基因 变体 |
| 收稿时间： | 2021-06-28 |
Detection of Feline Parvovirus and Mutation Analysis of VP2 Gene in Chengdu from 2017 to 2021 |
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| ZHOU Qun,YANG Yuan,SONG Xin,HE Xinyi,CAO Hui,ZHANG Bin.Detection of Feline Parvovirus and Mutation Analysis of VP2 Gene in Chengdu from 2017 to 2021[J].Acta Veterinaria et Zootechnica Sinica,2022,53(4):1303-1309. |
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| Authors: | ZHOU Qun YANG Yuan SONG Xin HE Xinyi CAO Hui ZHANG Bin |
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| Institution: | 1. College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China;2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education/Sichuan Provine, Chengdu 610041, China |
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| Abstract: | In order to investigate the prevalence and genetic variation of feline parvovirus (FPV) in domestic cats in Chengdu region. From 2017 to 2021, 168 diarrheal fecal samples were collected from domestic cats in Chengdu region of Sichuan province. FPV was detected in 168 samples, and 13 positive samples’nucleotide sequence of VP2 genes were obtained. The homology and variation of FPV nucleotide and amino acid (aa) sequences was analyzed by MegAlign software, and the phylogenetic tree was constructed by neighbor-joining method through MEGA 7.0. Of the 168 samples examined, 70.2% (118/168, 95% CI=62.7%-77.0%) samples were positive for FPV. Sequence homology analysis showed that the 13 full-length VP2 genes shared 97.1% to 100% homology to reference strains. The aa sequence of SMU-D18 and SMU-D14 strains were consistent with new CPV-2a from cats. Interestingly, the SMU-D46 strain showed a unique substitution from Ser (S) to Phe (F) at both 293aa and 297aa. Furthermore, phylogenetic analysis showed that the FPV strains from China and other countries clustered into three subgroups (G1, G2 and G3). The FPV isolates obtained in this study were located in G1 and G3, respectively. It was worth noting that the commercial FPV vaccine strain was located in G2, which had distantly related to the FPV strains circulating in China. The results demonstrated a very high infectious rate of FPV among cats in Chengdu region. The protective efficiency of the existing commercial vaccines should be re-evaluated as soon as possible. |
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| Keywords: | feline parvovirus molecular epidemiology VP2 gene variant |
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