| 利用50K芯片解析和田羊和策勒黑羊的遗传规律 |
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| 引用本文： | 张成龙,郑浪漫,刘春洁,高庆华,刘书东.利用50K芯片解析和田羊和策勒黑羊的遗传规律[J].畜牧兽医学报,2022,53(4):1051-1063. |
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| 作者姓名： | 张成龙 郑浪漫 刘春洁 高庆华 刘书东 |
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| 作者单位： | 1. 塔里木大学动物科学与技术学院, 阿拉尔 843300;2. 新疆生产建设兵团塔里木畜牧科技重点实验室, 阿拉尔 843300 |
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| 基金项目： | 国家自然科学基金(32060743)； |
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| 摘 要： | 旨在探究和田羊和策勒黑羊遗传规律的不同,筛选优异基因及其分子标记,推动新疆南疆绵羊品种遗传资源的开发和利用.本研究分别选取健康、成年和田母羊84只、策勒黑羊41只,采集耳组织,放入75％的酒精.基于酚氯仿法提取DNA,经电泳和核酸仪检测,制备Illumina Ovine SNP50芯片.用Plink1.07对基因组数据...
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| 关 键 词： | 50K芯片 遗传规律 选择信号 |
| 收稿时间： | 2021-06-07 |
Analysis of Genetic Law of Hetian and Qira Black Sheep Using Illumina Ovine SNP50 Chip |
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| ZHANG Chenglong,ZHENG Langman,LIU Chunjie,GAO Qinghua,LIU Shudong.Analysis of Genetic Law of Hetian and Qira Black Sheep Using Illumina Ovine SNP50 Chip[J].Acta Veterinaria et Zootechnica Sinica,2022,53(4):1051-1063. |
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| Authors: | ZHANG Chenglong ZHENG Langman LIU Chunjie GAO Qinghua LIU Shudong |
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| Institution: | 1. College of Animal Science and Technology, Tarim University, Alaer 843300, China;2. Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alaer 843300, China |
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| Abstract: | The aim of this study was to investigate the differences in genetic patterns between Hetian and Qira Black sheep, to screen superior genes and their molecular markers, and to promote the development and utilization of genetic resources of sheep breeds in southern Xinjiang. In this study, 84 healthy, adult Hetian ewes and 41 Qira Black sheep were selected, respectively, and ear tissues were collected and put into 75% alcohol. DNA was extracted based on phenol-chloroform method, and Illumina Ovine SNP50 was prepared by electrophoresis and nucleic acid instrumentation. For genomic data, quality control was performed with Plink1.07, and quality control criteria: exclusion of SNPs with detection rate less than 90%, minimum allele frequency (MAF)<5%, and Hardy-Weinberg equilibrium P<1×10-6. PCA populations of both breeds were classified, and HapFLK analysis was performed to take the SNPs corresponding to the top 1% of P values, and after sheep genome Oar_v4.0 annotation of genes, the GO and KEGG analyses were performed on the candidate genes. The results showed that: 1) Hetian sheep and Qira Black sheep differed in genetic structure after different degrees of selection and breeding, but they were not highly differentiated compared with the Tibetan sheep population. 2) A total of 167 candidate genes were obtained, and based on the differences in production performance between Hetian sheep and Qira Black sheep, the genetic patterns of the two breeds were resolved at the genomic level, and genes related to sheep wool growth were found to be KRT82 and KRT84, etc., wool color-related genes KIT, etc., growth and development-related genes AMHR2, SP1, OARDACVR1B, ADIPOQ, etc., and environmental adaptation-related genes PDGFRA, ADIPOQ, etc. Genome-level differences between Hetian and Qira Black sheep in different production traits were resolved by genomic selective sweeping, and it was found that the two breeds differed in wool quality traits, coat color, and tail type, while the differences in reproduction-related traits were small. In conclusion, the sheep genome microarrays was used to compare the differences in genetic structure of Hetian and Qira Black sheep and to search for the related superior genes, which would provide reference for the conservation of sheep germplasm resources and molecular selection and breeding in Southern Xinjiang region. |
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| Keywords: | 50K chip genetic pattern selection signal |
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