| 热带无爪螨主要变应原Blo t 5和Blo t 21融合表达质粒诱导小鼠产生IgG和调节性T细胞 |
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| 引用本文： | 罗应,彭美琪,肖正泮,罗承慧,王大勇,裴业春.热带无爪螨主要变应原Blo t 5和Blo t 21融合表达质粒诱导小鼠产生IgG和调节性T细胞[J].畜牧兽医学报,2022,53(4):1220-1230. |
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| 作者姓名： | 罗应 彭美琪 肖正泮 罗承慧 王大勇 裴业春 |
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| 作者单位： | 1. 海南大学生命科学学院, 海口 570228;2. 海南大学全健康研究院, 海口 570228 |
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| 基金项目： | 海南省自然科学基金高层次人才项目(320RC467)；;国家自然科学基金(31860726; 32160837)； |
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| 摘 要： | 旨在开发防治热带螨过敏症的兽用核酸疫苗，本试验通过对热带无爪螨主要变应原Blo t 5和Blo t 21的基因序列进行密码子优化，构建了热带螨主要变应原融合基因的真核表达载体pVAX1-PADRE-Blo t 5-21-CpG。为验证该真核表达载体的免疫效果，采用皮下注射，以100 μg/100 μL量的pVAX1-PADRE-Blo t 5-21-CpG核酸免疫BALB/c小鼠(n=6)，通过ELISA检测小鼠血清中特异性IgG、IgG1和IgG2a的水平变化；流式细胞术检测小鼠脾淋巴细胞中调节性T细胞的变化。结果显示，真核表达质粒pVAX1-PADRE-Blo t 5-21-CpG免疫小鼠后可诱导特异性IgG和IgG2a水平升高，且IgG2a/IgG1的比值升高，这暗示免疫后小鼠机体发生偏向Th1型的免疫反应。此外，该核酸疫苗可诱导小鼠产生较高水平的CD4+CD25+Foxp3+ T细胞，这表明该真核表达质粒免疫小鼠后可能会诱发免疫抑制。
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| 关 键 词： | 热带螨 变应原 真核表达载体 DNA疫苗 |
| 收稿时间： | 2021-07-16 |
Expression of Blomia tropicalis Major Allergens Blo t 5 and Blo t 21 Induced the Production of IgG and Regulatory T Cells in Mice |
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| LUO Ying,PENG Meiqi,XIAO Zhengpan,LUO Chenghui,WANG Dayong,PEI Yechun.Expression of Blomia tropicalis Major Allergens Blo t 5 and Blo t 21 Induced the Production of IgG and Regulatory T Cells in Mice[J].Acta Veterinaria et Zootechnica Sinica,2022,53(4):1220-1230. |
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| Authors: | LUO Ying PENG Meiqi XIAO Zhengpan LUO Chenghui WANG Dayong PEI Yechun |
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| Institution: | 1. School of Life Sciences, Hainan University, Haikou 570228, China;2. One Health Institute, Hainan University, Haikou 570228, China |
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| Abstract: | To develop DNA vaccine against Blomia tropicalis allergy, we constructed eukaryotic expression vector pVAX1-PADRE-Blo t 5-21-CpG of major allergen fusion gene of Blomia tropicalis by codon optimization of Blo t 5 and Blo t 21 gene sequences. To verify the immune effect of the eukaryotic expression vector, BALB/c mice were immunized with 100 μg/100 μL of pVAX1-PADRE-Blo t 5-21-CpG by subcutaneous injection (n=6)，and the serum levels of specific IgG, IgG1 and IgG2a were detected by ELISA. The changes of regulatory T cells in spleen lymphocytes of mice were detected by flow cytometry. The results showed that the eukaryotic expression plasmid pVAX1-PADRE- Blo t 5-21-CpG could induce the increase of specific IgG and IgG2a levels in mice, and the increase of IgG2a/IgG1 ratio suggested a bias towards a Th1 type immune response in mice after immunization. In addition, the plasmid pVAX1-PADRE-Blo t 5-21-CpG can induce higher levels of CD4+CD25+Foxp3+ T cells in mice, suggesting that the eukaryotic expression plasmid may induce immunosuppression. |
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| Keywords: | Blomia tropicalis allergen eukaryotic expression vector DNA vaccine |
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