Plasma Membrane Lipids Are the Powerful Components for Early Stage Aluminum Tolerance in Triticale |
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| Authors: | Tadao Wagatsuma Satoru Ishikawa Matsuo Uemura Wataru Mitsuhashi Takeshi Kawamura Md Shahadat Hossain Khan Keitarou Tawaraya |
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| Institution: | Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata, 997–8555 Japan;National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, 305–8604 Japan;Cryohiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, 020–8550 Japan |
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| Abstract: | The order of aluminum (Al) tolerance in triticale lines (ST2>ST22) after re-elongation in an Al-free 0.2 mM calcium (Ca) solution for 9 h (Ca period) following 1 h pretreatment with 20 μM Al (Al period) agreed with that after 24 h of Al treatment. Permeability of the plasma membrane (PM) of root-tip cells after the Ca period was significantly increased in Al-sensitive ST22. Al was accumulated more heavily in the root-tip portion of ST22 than in that of ST2, although similar amounts of malic and citric acid anions were released from both triticale lines. We established a new system examining lipid permeability using synthesized nylon-2,8 ultrathin and porous capsules trapped previously with 0.1% (w/v) methylene blue solution and coated thereafter with PM lipid isolated from root tips by a newly developed technique. Permeability of the PM lipid measured with time in 0.2 mM Ca with or without 50 μM Al photometrically ( A 680) was significantly greater in Al-sensitive ST22 after 5 min of Al treatment. This is the first report to directly show the primary and early role of PM lipid in Al tolerance in triticale. |
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| Keywords: | Al tolerance nylon capsule plasma membrane lipids release of organic acid anions triticale |
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