| 甜高粱组培苗生根培养的优化 |
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| 引用本文： | 葛玉红,谢鸿锴,韩佳宇.甜高粱组培苗生根培养的优化[J].南方农业学报,2012,43(2):155-159. |
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| 作者姓名： | 葛玉红 谢鸿锴 韩佳宇 |
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| 作者单位： | 广西大学农学院 |
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| 基金项目： | Cooperative and Special Project of Nanning City and Guangxi University(200901029B);Initiation Fund for Ph.D. holders at Guangxi University(X041054) |
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| 摘 要： | 【目的】优化能源作物甜高粱组培苗的生根培养体系，为遗传转化奠定基础。【方法】利用不同基本培养基（MS、1/2MS）对组培苗生根的影响进行筛选试验，得到较有利于生根的基本培养基；在最佳培养基中添加不同浓度的植物激素（IBA、NAA、ABT），综合根的质量、生根率、生根时间、生根苗的长势等筛选最佳生根培养基配方。【结果】筛选出最佳生根培养基组合：北甜三号（BT3）为1/2MS+2.0 mg/L IBA，辽甜三号（LT3）为1/2MS+3.0 mg/L IBA，且根粗、苗壮，两品种的生根率存在差异。此外，未加激素的MS和1/2MS培养基均无组培苗生根。【结论】在1/2MS上添加2.0 mg/L IBA或3.0 mg/L IBA分别为北甜三号、辽甜三号组培苗生根的最佳途径。
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| 关 键 词： | 甜高粱 组培苗生根 优化 植物激素 基本培养基 |
Optimization of media for in vitro root induction in different genotypes of sweet sorghum |
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| GE Yu-hong,XIE Hong-kai,HAN Jia-yu.Optimization of media for in vitro root induction in different genotypes of sweet sorghum[J].Journal of Southern Agriculture,2012,43(2):155-159. |
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| Authors: | GE Yu-hong XIE Hong-kai HAN Jia-yu |
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| Institution: | (Agricultural College,Guangxi University,Nanning 530005,China) |
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| Abstract: | Objective]The present experiment was conducted to optimize the root regeneration system in sweet sorghum in order to develop an effective genetic transformation protocol.Method]Different basic media(MS,1/2MS) and different concentrations of growth hormones(IBA,NAA and ABT) were evaluated and their effects on various factors such as root quality,rooting rate,rooting time,growth of rooted plantlets,etc.,were determined to obtain an optimum rooting medium.Result]The optimum rooting medium for the sweet sorghum variety BT3 and LT3 were found as 1/2MS +IBA(2.0 mg/L) and 1/2MS + IBA(3.0 mg/L),respectively.The regenerated roots were thick,and the rooted plantlets showed vigorous growth.The rooting rates varied in two varieties.Conclusion]It is concluded that best quality of roots can be regenerated on shoots using 1/2MS medium,supplemented with 2.0 or 3.0 mg/L IBA for variety BT3 and LT3,respectively. |
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| Keywords: | sweet sorghum plantlet rooting optimization plant hormone basic medium |
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