| 广西猪瘟病毒E2基因克隆及序列分析 |
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| 引用本文： | 陆增荣,张民秀,肖雄,王艺,刘芳,刘欢,卢冰霞,刘磊,郭旋,黎木兰,黄福标,黄伟坚.广西猪瘟病毒E2基因克隆及序列分析[J].南方农业学报,2012,43(4):528-531. |
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| 作者姓名： | 陆增荣 张民秀 肖雄 王艺 刘芳 刘欢 卢冰霞 刘磊 郭旋 黎木兰 黄福标 黄伟坚 |
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| 作者单位： | 广西大学动物科学技术学院,南宁530005广西丽原生物股份有限公司,南宁530001广西大学动物科学技术学院,南宁,530005 |
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| 基金项目： | 广西科学研究与技术开发计划项目(桂科攻1123007-3) |
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| 摘 要： | 目的]了解广西猪瘟病毒(CSFV)流行毒株的变异规律及其与疫苗株的基因差异,为正确选择猪瘟疫苗和防制广西猪瘟提供参考依据.方法]以广西本地的阳性猪瘟病料为研究对象,应用RT-PCR扩增CSFV的E2基因,经克隆、测序后用DNASTAR软件对其序列进行比对分析,并绘制遗传进化树.结果]从41份疑似猪瘟病料中共扩增出4个阳性样品的E2基因(1140 bp),经序列比对分析发现,扩增获得的4株CSFV毒株(GXGG1、GXLC1、GXLC2和GXNN1)与兔化弱毒株(HCLV)、Shimen强毒株的核苷酸同源性为82.1%~82.3%和82.9%~83.4%、其推导氨基酸同源性为88.4%~89.2%和88.9%~90.0%,4株毒株间的E2基因核苷酸及其推导氨基酸同源性分别为90.8%~99.6%和94.2%~99.5%,且均属于基因群Ⅱ;E2基因推导的氨基酸表明,E2蛋白空间结构及抗原结构的氨基酸位点693Cys、737Cys、792Cys、818Cys、828Cys、856Cys、833Pro、834Thr、837Arg均未发生变异,但其单抗识别位点G713E、N729D、K734R发生变异.结论]近年来广西CSFV流行毒株的变异未出现较大差异.与HCLV株、Shimen强毒株亲缘关系较远,但与Paderborn株、GXWZ02株亲缘关系较近.
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| 关 键 词： | 猪瘟病毒 E2基因 序列分析 同源性 氨基酸变异 广西 |
Cloning and sequencing of classical swine fever virus E2 gene in Guangxi |
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| LU Zeng-rong,ZHANG Min-xiu,XIAO Xiong,WANG Yi,LIU Fang,LIU Huan,LU Bing-xia,LIU Lei,GUO Xuan,LI Mu-lan,HUANG Fu-biao,HUANG Wei-jian.Cloning and sequencing of classical swine fever virus E2 gene in Guangxi[J].Journal of Southern Agriculture,2012,43(4):528-531. |
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| Authors: | LU Zeng-rong ZHANG Min-xiu XIAO Xiong WANG Yi LIU Fang LIU Huan LU Bing-xia LIU Lei GUO Xuan LI Mu-lan HUANG Fu-biao HUANG Wei-jian |
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| Institution: | 1(1College of Animal Science and Technology,Guangxi University,Nanning 530005,China;2Guangxi Liyuan Biological Corporation Ltd.,Nanning 530001,China) |
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| Abstract: | Objective]Variations regulation of classical swine fever virus(CSFV) prevalent strains in Guangxi and its difference with vaccine strain was studied in order to select a swine fever vaccine for controlling this disease in Guangxi.Method]The CSFV E2 gene in local positive diseased swine samples was amplified using RT-PCR,cloned into the pMD18-T vector and sequenced.The sequences were comparatively analyzed using DNASTART Software and heredity evolutionary tree was drawn.Result]Four positive strains(GXGG1,GXLC1,GXLC2 and GXNN1) of E2 gene were amplified from 41 CSF samples.The length of E2 gene was 1140 bp.The homologies of nucleotide sequence of 4 strains were 82.1-82.3% and 82.9-83.4%,and the homologies of deduced amino acid sequence were 88.4-89.2% and 88.9-90.0% with HCLV and Shimen strain,respectively.The homologies of nucleotide and amino acid sequence of 4 strains were 90.8-99.6% and 94.2-99.5%,respectively.These strains were classified into 2 groups,and the 4 strains belonged to the genotypeⅡ.No variation occurred at E2 protein structure and amino acid sites:693Cys,737Cys,792Cys,818Cys,828Cys,856Cys,833Pro,834Thr and 837Arg,however,the epitope(G713E,N729D,K734R) changed.Conclusion]No big variation happened to common CSFV strains during recent years in Guangxi,and they were not found close to HCLV and Shimen.Nevertheless,CSFV strains shared high homology with Paderborn and GXWZ02. |
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| Keywords: | classical swine fever virus(CSFV) E2 gene sequence analysis homology amino acid variation Guangxi |
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