| 22个非洲菊品种遗传多样性ISSR分析 |
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| 引用本文： | 钟海丰,黄宇翔,钟淮钦,黄敏玲.22个非洲菊品种遗传多样性ISSR分析[J].南方农业学报,2012,43(1):1-4. |
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| 作者姓名： | 钟海丰 黄宇翔 钟淮钦 黄敏玲 |
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| 作者单位： | 福建省农业科学院作物研究所/福建建省特色花卉工程技术研究中心,福州,350013 |
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| 基金项目： | 福建省科技重大专项项目(2010NZ0003-1);福建省农业科学院科技创新团队建设基金项目(STIF-Y06);福建省青年人才项目(2008F3023);福建省科技厅星火计划重点项目(2011S0057) |
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| 摘 要： | 目的]从分子水平上了解非洲菊主栽品种的亲缘关系及遗传多样性,为选配非洲菊杂交育种亲本提供参考.方法]设计100条ISSR引物,利用ISSR分子标记技术对外引的22个非洲菊品种进行遗传多样性及聚类分析.结果]筛选的9个ISSR引物共扩增出139条清晰带,其中多态性带127条,多态性比率91.37%.品种间的遗传相似系数在0.5095~0.8889,平均为0.7230.UPGMA聚类分析结果可将供试材料分为4组,其中第1组又可分为两个亚组.结论]非洲菊品种间具有较丰富的遗传多样性,花瓣及花心颜色可以作为区分非洲菊品种分类及分析亲缘关系的初步依据.
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| 关 键 词： | 非洲菊 ISSR 遗传多样性 聚类分析 |
Genetic diversity analysis of 22 Gerbera jamesonii cultivars using ISSR markers |
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| ZHONG Hai-feng, HUANG Yu-xiang , ZHONG Huai-qin, HUANG Min-ling.Genetic diversity analysis of 22 Gerbera jamesonii cultivars using ISSR markers[J].Journal of Southern Agriculture,2012,43(1):1-4. |
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| Authors: | ZHONG Hai-feng HUANG Yu-xiang ZHONG Huai-qin HUANG Min-ling |
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| Institution: | (Crop Research Institute, Fujian Academy of Agricultural Sciences / Fujian Engineering Research Center for Characteristic Floriculture, Fuzhou 350013, China) |
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| Abstract: | Objective]The present experiment was conducted to study the genetic diversity and relationship amongst different Gerbera jamesonii cultivars in order to screen parents for hybrid breeding. Method]Inter simple sequence repeat (ISSR) molecular markers were used to assess the genetic diversity amongst 22 G. jamesonii cultivars using 100 ISSR primers and cluster analysis was performed. Result]Out of 100 primers, 9 primers were selected and 139 reproducible DNA bands were amplified, of which 127 bands (91.37%) were polymorphic. The coefficient of genetic similarity in different cultivars ranged from 0.5909 to 0.8889 with an average of 0.7230, showing that there was wide genetic diversity amongst different cultivars. Unweighted pair group method with arithmetic mean (UPGMA) was used to draw a dendrogram, according to which the cultivars were clustered into four groups, and the first group could be divided into two subgroups. Conclusion]Wide genetic diversity amongst 22 G. jamesonii cultivars has been observed. Further, the morphological characteristics studies revealed that the color of petals and corolla may be used in preliminary classification and analysis of genetic relationship amongst various G. jamesonii cultivars. |
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| Keywords: | Gerbera jamesonii ISSR genetic similarity clustering analysis |
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