| 大孔吸附树脂—比色法测定番茄水提物中的总皂苷 |
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| 引用本文： | 蒋海英,陈思呈,李典鹏.大孔吸附树脂—比色法测定番茄水提物中的总皂苷[J].南方农业学报,2012,42(1):86-89. |
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| 作者姓名： | 蒋海英 陈思呈 李典鹏 |
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| 作者单位： | 广西壮族自治区中国科学院广西植物研究所;广西师范大学生命科学学院 |
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| 基金项目： | 广西科学基金应用基础研究专项项目(桂科基0991020);桂林市科学研究与技术开发计划项目(20100103-3) |
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| 摘 要： | 【目的】建立番茄水提物中总皂苷含量测定的方法，为进一步研究番茄皂苷奠定基础。【方法】成熟樱桃小番茄经打浆酶解后，所得清液经D-101大孔树脂吸附制得粗皂苷，以番茄皂苷A为标准品，以香草醛—冰醋酸+高氯酸体系作显色剂，于550 nm下测定总皂苷的含量。【结果】标准曲线在0.052～0.312 mg范围内线性关系良好（R2=0.9958）；平均加样回收率为99.53%，RSD=0.52%（n=6）；对5批次粗皂苷粉末中的总皂苷进行测定，其平均含量为（46.75±2.09）%。【结论】大孔吸附树脂—比色法操作简便、快速、准确，适用于测定番茄水提物中的总皂苷。
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| 关 键 词： | 番茄水提物 总皂苷 大孔吸附树脂—比色法 |
Determination of total saponin content in aqueous extract of tomatoes using macro-reticular resin colorimetric method |
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| JIANG Hai-ying,CHEN Si-cheng,LI Dian-peng.Determination of total saponin content in aqueous extract of tomatoes using macro-reticular resin colorimetric method[J].Journal of Southern Agriculture,2012,42(1):86-89. |
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| Authors: | JIANG Hai-ying CHEN Si-cheng LI Dian-peng |
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| Institution: | 1 * (1 Guangxi Institute of Botany, the Chinese Academy of Sciences, Guilin, Guangxi 541006, China; 2 College of Life Science, Guangxi Normal University, Guilin, Guangxi 541004, China) |
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| Abstract: | Objective]A method for determining total saponin content in aqueous extract of tomatoes was developed to facilitate studies on tomato saponins. Method]After zymohydrolysis, tomato slurry obtained by smashing was subjected to D-101 macroporous resin to get crude saponins. Esculeoside A was used as reference and vanillin-perchloric acid was applied as chromogenic reagent to determine total saponin content at 550 nm. Result]The standard curve of esculeoside A showed good linearity in the range of 0.052-0.312 mg with R2=0.9958, and the average recovery of added sample was 99.53% with 0.52%(n=6) RSD. Total saponin content obtained in five batches of crude saponin extraction was found to be 46.75±2.09%. Conclusion]The method developed in the present study was rapid, accurate and easy to be used,therefore would be good for determination of total sapnion content in water extract of tomatoes. |
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| Keywords: | tomato water extract total saponin macro-reticular resin-colorimetric method |
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