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| CpG寡核苷酸对IBDV VP2基因真核表达质粒免疫增效作用 | |
| 引用本文： | 王晓泉,姜平,丁铲,许家荣.CpG寡核苷酸对IBDV VP2基因真核表达质粒免疫增效作用[J].中国兽医学报,2004,24(2):114-116. |
| 作者姓名： | 王晓泉 姜平 丁铲 许家荣 |
| 作者单位： | 1. 南京农业大学,动物医学院农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095;中国农业科学院家禽所,江苏,扬州,225003 2. 南京农业大学,动物医学院农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 3. 中国农业科学院家禽所,江苏,扬州,225003 |
| 基金项目： | 江苏省农业新技术革命项目 (BG985 0 1-14 ) |
| 摘 要： | 以传染性法氏囊病病毒(IBDV)VP2蛋白基因表达质粒DNA为免疫原，以CpG的寡核苷酸(CpG-0DN)为免疫佐剂，肌肉注射于14日龄SPF鸡，1周后加强免疫1次，2次免疫后15d和21d分别测定血清ELISA抗体效价，并于免疫后21d用IBDV99儿强毒株攻毒和进行病理学观察。结果显示，(1)VP2基因重组质粒DNA与CpG共同免疫组的ELISA抗体水平明显高于VP2重组质粒免疫组；(2)IBD弱毒苗与VP2重组质粒免疫组抗体水平明显高于VP2重组质粒免疫组，且比VP2基因重组质粒DNA与CpG共同免疫组略高；(3)VP2基因重组质粒DNA与CpG共同免疫组及IBD弱毒苗与VP2重组质粒免疫组可明显降低IBDV强毒攻击后引起的急性发病率和死亡率。由此表明，CpG寡核苷酸对IBDV VP2蛋白基因真核表达质粒免疫具有明显增强作用，有很大的应用前景。 |
| 关 键 词： | 真核表达质粒 CpG寡核苷酸 免疫 传染性法氏囊病病毒 |
| 文章编号： | 1005-4545(2004)02-0114-03 |
| 修稿时间： | 2003年3月17日 |
Immune Response in Chicken Against VP2 Protein Eukaryoticex Expressing Plasmids of IBDV Enhanced with CpG ODN |
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| WANG Xiao-quan.Immune Response in Chicken Against VP2 Protein Eukaryoticex Expressing Plasmids of IBDV Enhanced with CpG ODN[J].Chinese Journal of Veterinary Science,2004,24(2):114-116. | |
| Authors: | WANG Xiao-quan |
| Institution: | WANG Xiao-quan~ |
| Abstract: | The present study was conducted to investigate the feasibility of two plasmids DNA carrying VP2 genes of the very virulent strain of IBDV(vvIBDV),pcDNA3.1-VP2 and pCI-VP2,and CpG ODN to protect chickens against infectious bursal disease virus(IBDV) infection.Fourteen-day-old chickens were intramuscularly injected with the plasmids alone or plus commercial attenuated IBD vaccine or synthetic CpG ODN twice at weekly intervals.Chickens at 5 weeks old were orally inoculated with vvIBDV strain 99J1 and observed for 7 days after challenge.Immunization with plasmid plus commercial attenuated IBD vaccine or CpG ODN conferred protection for 70% and 75% of chickens,respectively,as evidenced by the absence of clinical signs,mortality and bursal atrophy.About 10%-25% chickens vaccinated with commercial attenuated IBD vaccine or plasmid pcDNA3.1-VP2 or pCI-VP2 alone had clinical signs and died after challenge.The survived chickens exhibited varying degree of bursal atrophy.Enzyme-linked immunosorbent assay(ELISA) antibody titers in chickens vaccinated the constructs DNA plus live vaccine or CpG ODN were significantly higher than those inoculated with the constructs or the live vaccine alone.These results suggest that co-administration of the constructed plasmid pcDNA3.1-VP2 or pCI-VP2 with CpG ODN or commercial attenuated IBD vaccine could protect efficiently the chicken from direct vvIBDV challenge. |
| Keywords: | IBDV VP2 eukaryoticex expressing plasmid CpG ODN |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |