| 拟南芥甘油二酯激酶(AtDGK7)基因抗逆的生理作用 |
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| 引用本文： | 袁苑,刘庆周,武玉叶,李德全.拟南芥甘油二酯激酶(AtDGK7)基因抗逆的生理作用[J].农业生物技术学报,2009,17(1):90-97. |
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| 作者姓名： | 袁苑 刘庆周 武玉叶 李德全 |
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| 作者单位： | 山东农业大学生命科学学院,作物生物学罔家重点实验室,泰安271018 |
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| 基金项目： | 山东农业大学科技创新基金，教育部长江学者与创新团队发展计划 |
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| 摘 要： | 构建表达载体PBI121-AtDGK7，转化拟南芥（Arabidopsis thaliana）获得过表达转基因植株。通过抗逆生理指标测定、ABA、NaCl和甘露醇抗性试验, 以及半定量RT-PCR方法，对拟南芥甘油二酯激酶(AtDGK7)基因的抗逆生理功能进行初步研究。结果表明，过表达AtDGK7转基因拟南芥对高浓度ABA的敏感性比野生型要低，能更好地抵御盐胁迫。通过测定脯氨酸(Pro)和丙二醛(MDA)含量以及超氧物歧化酶(SOD)活性，进一步证实过表达AtDGK7转基因拟南芥植株的耐低温能力增强。
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| 关 键 词： | 甘油二酯激酶基因 转基因拟南芥植株 功能分析 逆境胁迫 抗逆性 |
| 收稿时间： | 2008-6-3 |
| 修稿时间： | 2008-7-7 |
Stress Physiological Roles of Diacylglycerol Kinase (AtDGK7) Gene in Arabidopsis thaliana |
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| YUAN Yuan,LIU Qing-zhou,WU Yu-ye,LI De-quan.Stress Physiological Roles of Diacylglycerol Kinase (AtDGK7) Gene in Arabidopsis thaliana[J].Journal of Agricultural Biotechnology,2009,17(1):90-97. |
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| Authors: | YUAN Yuan LIU Qing-zhou WU Yu-ye LI De-quan |
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| Abstract: | Transgenic AtDGK7 plants were obtained by constructing PBI121-AtDGK7 expression vector and transformating it into Arabidopsis thaliana. And the stress-resistant function of diacylglycerol kinase (AtDGK7) gene in Arabidopsis thaliana was researched by stress resistance determination, ABA, NaCl and mannitol resistance experiment and RT-PCR. The results showed that the AtDGK7-overexpressed Arabidopsis thaliana plants were less sensitive to high concentration ABA and displayed normal germination of seeds. Under salt stress, the AtDGK7-overexpressed Arabidopsis thaliana plants grew more robust than that of the wild-type Arabidopsis thaliana plants , and could resist the salt stress. Meanwhile, the free proline and malonaldehyde contents, and superoxide dismutase activities were determined. And the results showed that the low temperature-resistant of the AtDGK7-overexpressed Arabidopsis thaliana plants were proved. |
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