| 菜豆（油豆角）苗期抗炭疽病防御酶活性及HRGP含量表达分析 |
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| 引用本文： | 曾棱男,肖子恒,李成雁,张婷,胡国富.菜豆（油豆角）苗期抗炭疽病防御酶活性及HRGP含量表达分析[J].中国蔬菜,2012,1(12):47-51. |
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| 作者姓名： | 曾棱男 肖子恒 李成雁 张婷 胡国富 |
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| 作者单位： | 东北农业大学生命科学学院，黑龙江哈尔滨 150030 |
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| 基金项目： | 黑龙江省博士后基金项目(LRB07-3) |
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| 摘 要： | 以感病菜豆（油豆角）品种老来少和高抗品种大油豆为试材，在菜豆炭疽病菌侵染不同时间（0、3、6、9、12 d）内测定两者叶片中5种防御酶（几丁质酶、β-1，3-葡聚糖酶、SOD、POD和LOX）活性及富含羟脯氨酸糖蛋白（HRGP）含量并进行比较。结果表明：接种后抗病品种的防御酶活性和HRGP含量均有不同程度的变化，酶活性提高幅度较大的时期因酶种类而异，但都早于感病品种|感病品种防御酶活性升高较慢且较抗病品种滞后。与感病品种相比，抗病品种POD活性变化幅度最大，而HRGP含量变化幅度最小。β-1，3-葡聚糖酶活性测定结果表明，抗病品种接种前酶活性高于感病品种，接种后感病和抗病品种酶活性变化趋势相同。
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| 关 键 词： | 炭疽病 菜豆 防御酶 酶活性 HRGP含量 |
Analysis on Expression of Anthracnose Defense Enzyme Activity and HRGP Content in Phaseolus vulgaris L.at Seedling Stage |
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| ZENG Ling-nan,XIAO Zi-heng,LI Cheng-yan,ZHANG Ting,HU Guo-fu.Analysis on Expression of Anthracnose Defense Enzyme Activity and HRGP Content in Phaseolus vulgaris L.at Seedling Stage[J].China Vegetables,2012,1(12):47-51. |
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| Authors: | ZENG Ling-nan XIAO Zi-heng LI Cheng-yan ZHANG Ting HU Guo-fu |
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| Institution: | Life Science College of Northeast Agricultural University，Harbin 150030，Heilongjiang，China |
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| Abstract: | The susceptible bean(Phaseolus vulgaris L.var.chinensis Hort.)cultivar’Laolaishao’ and resistant cultivar’Dayoudou’were taken as testing materials.The activities of 5 kinds defense enzymes(chitinase,beta-1,3-glucanase,SOD,POD and LOX)and HRGP content were detected and analyzed after the 2 cultivars infected by Anthracnose in different times(0,3,6,9,12 d).The results showed that content of HRGP and the enzyme activities of chitinase,SOD,POD and LOX in resistant cultivar markedly increased with the increase of time course of infection and the disease expansion.The peak of their activities appeared earlier than in susceptible one.Compared with the susceptible cultivars,the activity of POD in resistant cultivar changed most significantly,while the content of HRGP changed with minimum amplitude.And the results of testing enzyme activity of beta-1,3-glucanase showed that the enzyme activity of resistant cultivar in 0 d was higher than susceptible cultivar,while after inoculation its enzyme activity of resistant cultivar change tendency was the same as the susceptible cultivar. |
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| Keywords: | Anthracnose Bean Defense enzyme Enzyme activity HRGP content |
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