Identification of the spontaneous 7BS/7RL intergenomic translocation in one F1 multigeneric hybrid from the Triticeae tribe |
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| Authors: | A Carvalho A Martín J S Heslop-Harrison H Guedes-Pinto and J Lima-Brito |
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| Institution: | Institute of Biotechnology and Bioengineering (IBB), Centre of Genetics and Biotechnology (CGB), University of Trás-os-Montes and Alto Douro (UTAD), Ap. 1013, 5000-801 VilaReal, Portugal, E-mail:;;Instituto de Agricultura Sostenible (CSIC), Apartado 4084, 14080 Córdoba, Spain;;Department of Biology, University of Leicester, Leicester, LE1 7RH, UK |
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| Abstract: | The F1 AABBRHch hybrids studied here were produced by crosses between the Portuguese triticale cultivar 'Douro' (AABBRR) and the tritordeum line HT9 (AABBHchHch). Fluorescent in situ hybridization performed with genomic DNA probes genomic in situ hybridization (GISH) from rye and Hordeum chilense allowed the unequivocal parental genomes discrimination in all hybrids. Among 55 plants, one presented a spontaneous wheat–rye translocation which was successfully detected after GISH. Recombinant chromosomes identification was made after reprobe with pTa71 and pSc119.2. Nine rDNA loci were detected by pTa71 and pSc119.2 identified the chromosome arms involved in the translocation, after comparing the observed hybridization patterns with those described by several authors. We identified the spontaneous wheat–rye translocation as being the 7BS/7RL. Many wheat–rye translocations have been found (e.g. 1BL.1RS and 1AL.1RS), but as far as we know, this is the first time that this translocation is reported. We considered it helpful for wheat breeding programmes as it could provide the transference of interesting agronomic characteristics from rye (e.g. leaf rust resistance) to wheat. |
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| Keywords: | fluorescent in situ hybridization intergenomic spontaneous translocation triticale × tritordeum hybrids |
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