| 高通量筛选研究茯砖茶降脂功效 |
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| 引用本文： | 傅冬和,刘仲华,黄建安,龚雨顺,陈金华.高通量筛选研究茯砖茶降脂功效[J].茶叶科学,2006,26(3):209-214. |
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| 作者姓名： | 傅冬和 刘仲华 黄建安 龚雨顺 陈金华 |
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| 作者单位： | 教育部茶学重点实验室,湖南,长沙,410128;教育部茶学重点实验室,湖南,长沙,410128;教育部茶学重点实验室,湖南,长沙,410128;教育部茶学重点实验室,湖南,长沙,410128;教育部茶学重点实验室,湖南,长沙,410128 |
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| 摘 要： | 寻找高效天然的降脂减肥药物是当前研究的热点之一,高通量药物筛选是近年发展起来的药物筛选新方法。将茯砖茶水提液分别用三氯甲烷、乙酸乙酯、正丁醇萃取,制得三氯甲烷层、乙酸乙酯层、正丁醇层及水层样品,茶渣用95%的乙醇提取,均采用过氧化物酶增殖体活化受体(PPARα、PPARγ、PPARδ)、肝X受体(LXR)、法尼酯衍生物X受体(FXR)及前脂肪细胞(3T3-L1)模型进行高通量药物筛选研究茯砖茶的降脂减肥功效,结果显示茯砖茶各部位对PPARγ、PPARδ有激活作用,对FXR有抑制作用,对PPRAα、3T3-L1作用不明显,证明茯砖茶有多条降脂途径,降脂减肥功能较强,有望开发成天然的降脂药物,其降脂有效成分还有待进一步研究。
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| 关 键 词： | 茯砖茶 过氧化物酶增殖体活化受体 肝X受体 法尼酯衍生物X受体 前脂肪细胞 高通量药物筛选 |
| 文章编号： | 1000-369X（2006）03-209-06 |
| 收稿时间： | 2006-02-13 |
| 修稿时间： | 2006-04-10 |
Research of FUZHUAN Tea's Therapy for Hyperlipidemia by High-Throughput Screening |
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| FU Dong-he,LIU Zhong-hua,HUANG Jianan,GONG Yu-shun,CHEN Jin-hua.Research of FUZHUAN Tea's Therapy for Hyperlipidemia by High-Throughput Screening[J].Journal of Tea Science,2006,26(3):209-214. |
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| Authors: | FU Dong-he LIU Zhong-hua HUANG Jianan GONG Yu-shun CHEN Jin-hua |
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| Institution: | The Key Laboratory of Tea Science of National Ministry of Education, Changsha 410128, China |
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| Abstract: | Searching high effective and natural medicines for therapy for hyperlipidemia is one of the focus of research.High-Throughput Screening is a kind of new technique of medicine screening.The water extractions of FUZHUAN Tea were extracted by chloroform,ethyl acetate,1-butanol separately.The samples were used to test their function of adjusting fat metabolism of FUZHUAN Tea by the models of PPARα,PPARγ,PPARδ,LXR,FXR and 3T3-L1 of High-Throughput Screening.The results showed that FUZHUAN Tea could activate PPARγ,PPARδ and restrain FXR.Its effect to PPRAα and 3T3-L1 was not obvious.FUZHUAN Tea was effective in therapy for hyperlipidemia.The active components need to be separated later. |
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| Keywords: | Fuzhuan Tea PPARα PPARγ PPARδ LXR FXR 3T3-L1 high-Throughput Screening |
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