Short-Term Storage of Semen of Rainbow Trout: Interactions of Time,Antibiotic, and Activator |
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| Authors: | Hamid Niksirat Koorosh Sarvi Asghar Abdoli Saeed Hajirezaee |
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| Institution: | 1. Department of Biodiversity and Ecosystem Management, Environmental Sciences Research Institute , Shahid Beheshti University , Tehran, Iran h_niksirat@yahoo.com;3. Department of Fisheries, Faculty of Natural Resources and Marine Sciences , Tarbiat Modares University , Mazandaran, Iran;4. Department of Biodiversity and Ecosystem Management, Environmental Sciences Research Institute , Shahid Beheshti University , Tehran, Iran;5. Department of Fisheries and Environmental Sciences, Faculty of Natural Resources , University of Tehran , Karaj, Iran |
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| Abstract: | This study was designed to examine the main effects and interactions of time, presence of antibiotics, and type of sperm activators on the fertilization capacity (eyeing rate) of refrigerated semen of rainbow trout, Oncorhynchus mykiss. The semen samples were stored in the presence or absence of 250 IU ml?1 penicillin and 250 μg ml?1 streptomycin sulfate. Freshwater and a saline solution were used as sperm activators. The semen samples were stored at 2–3°C and fertilized after 0, 6, 8, 12, 19, and 25 days of storage. Fertilizing capacities of semen samples stored in the presence of antibiotics (63.8 ± 5.6%) were significantly (p < 0.05) higher than those stored in the absence of antibiotics (46.2 ± 6.7%). Also, the fertilizing capacities of stored semen samples activated using saline solution (70.7 ± 5.7%) had significantly (p < 0.05) higher values than those activated using freshwater (39.3 ± 5.9%). Semen samples stored in the absence of antibiotics completely lost fertilizing capacity within 19 days of storage. After 25 days of storage in the presence of antibiotics, induction of fertilization using freshwater and saline solution resulted in 0% and 79.8 ± 1.7% fertility, respectively. |
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| Keywords: | Oncorhynchus mykiss activator antibiotic semen storage |
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