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| 西南五省区及非洲野生狗牙根种质基于SRAP标记的遗传多样性分析 | |
| 引用本文： | 凌瑶,张新全,齐晓芳,周莹洁,刘伟,马啸,陈仕勇.西南五省区及非洲野生狗牙根种质基于SRAP标记的遗传多样性分析[J].草业学报,2010,19(2):196-203. |
| 作者姓名： | 凌瑶 张新全 齐晓芳 周莹洁 刘伟 马啸 陈仕勇 |
| 作者单位： | 四川农业大学动物医学院,四川,雅安,652014;四川农业大学草业科学系,四川,雅安,625014;四川农业大学草业科学系,四川,雅安,625014 |
| 基金项目： | 科技部973项目，教育部新世纪优秀人才支持计划 |
| 摘 要： | 利用ＳＲＡＰ分子标记技术，对采自中国西南５省区的４４份野生狗牙根及８份非洲狗牙根材料进行遗传多样性研究。结果表明，１８对引物组合共得到扩增总条带２３６条，多态性条带数２０６条，平均每对引物扩增出１１．４条带，多态性位点百分率为８７．２９％，材料间的遗传相似系数范围为０．５６９～０．９２９，平均ＧＳ值为０．７２３。聚类分析结果表明，５２份材料可聚为５类；基于Ｓｈａｎｎｏｎ多样性指数估算了８个狗牙根生态地理类群内和类群间的遗传分化，类群内的遗传变异占总变异的６３．８１％，类群间的遗传变异占总变异的３６．１９％，表明这些抗源材料遗传差异较大，各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性。 |
| 关 键 词： | 狗牙根 SRAP 遗传多样性 聚类 |
| 收稿时间： | 1900-01-01; |
Genetic diversity of wild Cynodon dactylon germplasm from five province of Southwest China and Africa detected by SRAP markers |
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| LING Yao,ZHANG Xin-quan,QI Xiao-fang,ZHOU Ying-jie, LIU Wei,MA Xiao,CHEN Shi-yong.Genetic diversity of wild Cynodon dactylon germplasm from five province of Southwest China and Africa detected by SRAP markers[J].Acta Prataculturae Sinica,2010,19(2):196-203. |
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| Authors: | LING Yao ZHANG Xin-quan QI Xiao-fang ZHOU Ying-jie LIU Wei MA Xiao CHEN Shi-yong |
| Abstract: | Sequence-related amplified polymorphism (SRAP) markers were used to detect the genetic diversity of 44 wild accessions of Cynodon dactylon collected from Sichuan, Chongqing,Yunnan, Guizhou and Tibet of China, and 8 wild accessions of C. dactylon collected from Africa. The results showed that eighteen primer pairs produced 206 polymorphic bands, averaged 11.4 bands per primer pair. The percentage of polymorphic bands in average was 87.29%.The Nei's genetic similarity coefficient of the tested accessions ranged from 0.569 to 0.929, and the average Nei's coefficient was 0.723.The 54 accessions were classified into five major groups: Ⅰ, Ⅱ, Ⅲ, Ⅳand Ⅴ by cluster analysis using UPGMA, which showed significant relationship with the origin regions of accessions. Genetic differentiation between and within eight eco-geographical groups of C. dactylon was estimated by Shannon's diversity index, which showed that 63.81% genetic variance existed within group, and 36.19% genetic variance was among groups, indicating rather large genetic distances among those germplasm,and there was a correlation between genetic differentiation and eco-geographical habits among the groups. |
| Keywords: | SRAP Cynodon dactylon SRAP genetic diversity clustering |
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