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| 我国南方某省猪繁殖与呼吸综合症病毒分离株分子流行病学分析 | |
| 引用本文： | 吴发兴,解生亮,张燕霞,张志,李晓成,刘道新,黄保续.我国南方某省猪繁殖与呼吸综合症病毒分离株分子流行病学分析[J].中国动物检疫,2008,25(8):23-25. |
| 作者姓名： | 吴发兴 解生亮 张燕霞 张志 李晓成 刘道新 黄保续 |
| 作者单位： | 1. 中国动物卫生与流行病学中心,山东青岛,266032 2. 中国动物卫生与流行病学中心,山东青岛,266032;东北农业大学动物医学学院,黑龙江哈尔滨,150030 3. 湖南省兽医总站,湖南长沙,410007 |
| 摘 要： | 根据GenBank上发表的猪繁殖与呼吸综合征病毒(PRRSV)VR-2332的基因序列设计并合成了一对针对ORF5基因的引物,通过RT-PCR方法对我国南方某省部分地区分离的14个PRRSV分离株进行扩增,获得了大约773bp的DNA片断,将目的片断与pMD18-T载体进行连接并测序。应用DNAStar分析所测序列,并与LV、VR-2332、CH-1a、BJ-4、HB-1、HB-2、JX-1等毒株的序列进行比较。核苷酸序列分析表明:14株病毒的与JX-1ORF5基因同源性高达99.0%～99.8%,与CH-1a、HB-1、HB-2同源性达到91.4%～94.9%,与VR-2332、BJ-4同源性达到86.1%～87.6%,与LV同源性仅为55.2%～55.7%。氨基酸序列分析表明:与JX-1同源性高达97.5%～99%;与VR-2332、CH-1a、BJ-4、HB-1、HB-2同源性达到85.6%～94.5%;与LV同源性仅为55.7%～56.2%。可知这14株病毒与JX-1遗传关系较近,可归属同一基因亚群。 |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF5基因 进化树 分子流行病学 |
Molecular Epidemiological Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates in South China |
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| WU Faxing,XIE Shengliang,ZHANG Zhi,Zhang Yanxi,LI Xiaocheng,LIU Daoxin,HUANG Baoxu.Molecular Epidemiological Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates in South China[J].China Journal Of Animal Quarantine,2008,25(8):23-25. | |
| Authors: | WU Faxing XIE Shengliang ZHANG Zhi Zhang Yanxi LI Xiaocheng LIU Daoxin HUANG Baoxu |
| Institution: | 1(1.China Animal Health and Epidemiology Centre,Qingdao 266032;2.Northeast Agricultural University,College of Veterinary Medicine,Harbin 150030;3.Veterinary Station of Hunan Province,Changsha 410007) |
| Abstract: | A pair of primers specific to the ORF5 gene were designed and synthesized according to the published genome sequence of PRRSV VR-2332 on Genbank and an RT-PCR was developed to amplify 14 PRRSV isolates from parts of Hunan province,obtaining DNA fragments of approximetely 773bp.The target fragment was connected to pMD18-T vector and sequenced.The sequence was analysed by menas of DNA star software and compared with those of LV,VR-2332,CH-1a,JB-4,HB-1,HB-2,JX-1 strains.Nucleotide sequence analysis showed that the ORF-5 gene holomology of the 14 isolates was upto 99.0%-99.8% with JX-1;91.4%-94.9% with CH-1a,HB1 and HB2;86.1%-87.6% with VR-2332,BJ-4,and only 55.2%-55.7% with LV and the amino acid sequence analysis showed that the their homology was 97.5%-99% with JX-1;85.6%-94.5% with VR-2332,CH-1a,BJ-4,HB1 and HB2;and only 55.7%-56.2% with LV.It was suggested that the PRRSV 14 isolates are closely related to JX-1 genotically and can be grouped in the same subtype. |
| Keywords: | porcine reproductive and respiratory syndrome virus ORF5 genes phylogenetic tree molecular epidemiology |
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