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Increased resistance to white spot syndrome virus in Procambarus clarkii by injection of envelope protein VP28 expressed using recombinant baculovirus |
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| Authors: | Huahua Du Zirong Xu Xiaofeng Wu Weifen Li Wei Dai |
| Institution: | aKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou-310029, PR China bLab of Bioresource and Biotechnology, College of Animal Sciences, Zhejiang University, Hangzhou-310029, PR China |
| Abstract: | In order to investigate whether protein structure has an effect on protective effect of envelope protein of WSSV, VP28 protein was expressed both in Escherichia coli (pET-VP28) and insect (BmN) cells (BmNPV-VP28). The baculovirus (BmNPV) expression system was used to obtain correctly folded VP28 protein. Procambarus clarkii crayfish were intramuscularly injected with lysates of cells infected with recombinant pET-VP28 and BmNPV-VP28, respectively, and then challenged by intramuscular injection of WSSV to assess the duration of protection. The crayfish injected with BmNPV-VP28 showed generally lower mortality rates when compared to crayfish injected with pET-VP28, resulting in relative percent survivals of 92% and 39%, respectively, when compared to the control groups injected with empty vectors BmNPV and pET-30a. These results showed that VP28 protein produced in BmN cells gave much better protection than VP28 protein produced in E. coli. |
| Keywords: | White spot syndrome virus VP28 Protection Procambarus clarkii Crayfish |
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