| 牛分枝杆菌与鸟型分枝杆菌2型重组蛋白Ag85b的血清学交叉反应研究 |
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| 引用本文： | 朱婷,王华南,刘慧芳,于申业,王秀梅,陈莉苹,司微,赵海玲,刘思国.牛分枝杆菌与鸟型分枝杆菌2型重组蛋白Ag85b的血清学交叉反应研究[J].中国预防兽医学报,2012,34(3):223-226. |
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| 作者姓名： | 朱婷 王华南 刘慧芳 于申业 王秀梅 陈莉苹 司微 赵海玲 刘思国 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/动物细菌病研究室,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目(2012CB518801);国家科技基础性工作专项(2008FY210200) |
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| 摘 要： | 牛分枝杆菌(M.bovis)是引起牛结核病的常见病原,而鸟分枝杆菌(M.avium)2型则通常交叉感染牛,但不会导致严重的病变.为鉴定M.bovis和M.avium的免疫交叉反应情况,本研究分别以M.bovis和M.avium 2型的基因组为模板,扩增ag85b基因,分别构建了M.bovis和M.avium的原核和真核表达重组质粒进行表达.以原核表达纯化的两种重组蛋白Ag85b (rAg85b)分别作为包被抗原,交叉检测两种真核重组质粒免疫豚鼠制备的抗血清.结果表明,原核表达的M.bovis和M.avium rAg85b与真核重组质粒免疫豚鼠制备的两种抗血清之间存在较强的免疫交叉反应.研究结果揭示M.bovis和M.avium的Ag85b蛋白存在很强的血清学交叉反应,这将严重干扰M.bovis Ag85b作为候选疫苗的免疫监测.
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| 关 键 词： | 牛结核分枝杆菌 鸟型分枝杆菌 ag85b基因 交叉反应 |
Immune cross-reaction of recombinant proteins Ag85b between Mycobacterium bovis and Mycobacterium avium serotype 2 |
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| ZHU Ting
,
WANG Hua-nan
,
LIU Hui-fang
,
YU Shen-ye
,
WANG Xiu-mei
,
CHEN Li-ping
,
SI Wei
,
ZHAO Hai-ling
,
LIU Si-guo.Immune cross-reaction of recombinant proteins Ag85b between Mycobacterium bovis and Mycobacterium avium serotype 2[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(3):223-226. |
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| Authors: | ZHU Ting WANG Hua-nan LIU Hui-fang YU Shen-ye WANG Xiu-mei CHEN Li-ping SI Wei ZHAO Hai-ling LIU Si-guo |
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| Institution: | (Division of Animal Bacteriosis,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | Mycobacterium bovis is a common pathogen to cause bovine tuberculosis in cattle,but crossing infection of Mycobacterium avium is the normal cases in cattle.To identify serum crossing reactions of the 2 bacteria,the ag85b genes were amplified by PCR from genomes of M.bovis and M.avium serotype 2,respectively,and subcloned into pET28a and pcDNA3.1(pcDNA-Ag85b).Guinea pigs were immunized with pcDNA-Ag85b of M.bovis and M.avium,respectively,and the prepared anti-Ag85bs were used to analyze the cross-reactions of the two different Mycobacteria by ELISA with recombinant Ag85b proteins as coating antigens expressed in E.coli.The results shown that M.bovis and M.avium had strong cross-reactions between the two kinds of Ag85b proteins,which provided evidence that M.avium infection would interfere the immunologic surveillance to the use of vaccines containing Ag85b in cattle herd. |
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| Keywords: | Mycobacterium bovis Mycobacterium avium ag85b gene immune cross-reaction |
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