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| 血清1型鸭疫里默氏杆菌灭活油乳剂疫苗的研制 | |
| 引用本文： | 王小兰,胡青海,童永亮,韩先干,丁铲,于圣青.血清1型鸭疫里默氏杆菌灭活油乳剂疫苗的研制[J].中国预防兽医学报,2012,34(4):313-316. |
| 作者姓名： | 王小兰 胡青海 童永亮 韩先干 丁铲 于圣青 |
| 作者单位： | 1. 中国农业科学院上海兽医研究所,上海,200241 2. 中国农业科学院上海兽医研究所,上海200241;西南农业大学动物科技学院,重庆400716 3. 中国农业科学院上海兽医研究所,上海200241;国家家禽工程技术中心,上海200331 |
| 基金项目： | 上海市科技兴农重点攻关项目[沪农科攻字(2009)第5-3号];公益性行业(农业)科研专项经费项目(201003012) |
| 摘 要： | 为研究血清1型鸭疫里默氏杆菌(R.anatipestifer,RA)的灭活油乳剂疫苗,本研究将10株血清1型RA的临床分离株经腿部肌肉注射7日龄雏鸭进行动物体内复壮,结果表明所有被测菌株均具有较强的毒力,易感雏鸭致死率100％.对其中3株RA分离株CH3、WJ4和YL4的毒力测定结果表明其半数致死量(LD50)分别为2×108 cfu、3.25×108 cfu和2.37×106 cfu.选取5株RA分离株分别制备灭活油乳剂疫苗,分别于5日龄和18日龄对樱桃谷鸭进行两次免疫后,免疫鸭能够产生高水平的RA特异性抗体,对2 LD50 WJ4或CH3攻毒产生很好的保护效果,其中由CH3、CQ3和YXb12制备的灭活油乳剂疫苗对攻毒的保护率高达100％. |
| 关 键 词： | 鸭疫里默氏杆菌 油乳剂灭活疫苗 ELISA |
Study on the inactivated oil-emulsion vaccine of serotype 1 Riemerella anatipestifer |
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| WANG Xiao-lan , HU Qing-hai , TONG Yong-liang , HAN Xian-gan , DING Chan , YU Sheng-qing.Study on the inactivated oil-emulsion vaccine of serotype 1 Riemerella anatipestifer[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(4):313-316. | |
| Authors: | WANG Xiao-lan HU Qing-hai TONG Yong-liang HAN Xian-gan DING Chan YU Sheng-qing |
| Institution: | 1,2(1.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China; 2.China National Engineering Technology Research Centre for Poultry,Shanghai 200331,China; 3.College of Animal Science and Technology,Southwest Agricultural University,Chongqing 400716,China) |
| Abstract: | Riemerella anatipestifer infection is a contagious disease of ducks,goose,turkeys and various other birds,and has caused the highest mortality among duck diseases.Serotype 1 is one of the most prevalent serotype of R.anatipestifer infection.To efficient control the infection,we developed the inactivated oil-emulsion vaccines of serotype 1 R.anatipestifer.In this study,ten isolates of serotype 1 R.anatipestifer were injected intramuscularly into 7-day-old Cherry valley ducklings to restore the bacterial virulence and the bacteria re-isolated from the inoculated ducklings were used as seed bacteria.The median lethal dose(LD50) of isolates CH3,WJ4 and YL4 were determined as 2×108 cfu,3.25×108 cfu and 2.37×106 cfu respectively.Five isolates of the R.anatipestifer were used to prepare inactivated oil-emulsion vaccines,respectively.Cherry valley ducklings were immunized twice subcutaneously at 5 and 18-day-old,respectively,and all the ducklings developed high titers of specific antibodies against R.anatipestifer.The ducklings immunized with CH3,CQ3 or YXb12 vaccine were 100% protected from the challenges of 2LD50 of WJ4 or CH3. |
| Keywords: | Riemerella anatipestifer oil-emulsion vaccine ELISA |
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