| 毛乌素不同类型沙地油蒿种群遗传分化的ISSR分析 |
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| 引用本文： | 高凤月,朴顺姬,王大川,包萨如拉,许庆杰,高占成.毛乌素不同类型沙地油蒿种群遗传分化的ISSR分析[J].中国草地学报,2012,34(1):31-36. |
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| 作者姓名： | 高凤月 朴顺姬 王大川 包萨如拉 许庆杰 高占成 |
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| 作者单位： | 1. 内蒙古大学生命科学学院/内蒙古草地生态学重点实验室-省部共建国家重点实验室培育基地,内蒙古呼和浩特,010021
2. 毛乌素沙地治沙研究中心,内蒙古,017300 |
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| 基金项目： | 内蒙古自然科学基金，国家自然科学基金 |
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| 摘 要： | 利用12对微卫星引物对毛乌素沙地油蒿种群的遗传分化、基因流进行分析,采用Nei’s指数和Shannon指数估算3个不同类型沙地种群的遗传多样性,计算种群相似系数和遗传距离,利用遗传距离构建系统树。结果表明:(1)种群遗传多样性分析显示,油蒿种群多态位点百分率为100%,说明油蒿种群基因组ISSR具有较高的多样性。(2)在遗传距离为6.0处可将184个样株大致分为三类,涵盖了135个样株,占采样总量的73.34%。结合样地定位记录,聚类结果与样株空间分布具有一致性,即可分为流动沙地、固定沙地、半固定沙地油蒿种群。(3)流动沙地与固定、半固定沙地油蒿种群间的基因流较小,分别为0.7929和0.6848,半固定与固定沙地油蒿种群间存在着较高的基因流,为6.2930。半固定与固定沙地种群间的遗传一致度较高,为93.77%;流动沙地种群Shannon指数低。
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| 关 键 词： | 油蒿 遗传多样性 遗传分化 ISSR |
Genetic Differentiation of Artemisia ordosica Populations in Different Types of Sandy Land of Maowusu Based on ISSR |
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| GAO Feng-yue
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PIAO Shun-ji
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WANG Da-chuan
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BAO Sarula
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XU Qing-jie
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GAO Zhan-cheng.Genetic Differentiation of Artemisia ordosica Populations in Different Types of Sandy Land of Maowusu Based on ISSR[J].Chinese Journal of Grassland,2012,34(1):31-36. |
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| Authors: | GAO Feng-yue PIAO Shun-ji WANG Da-chuan BAO Sarula XU Qing-jie GAO Zhan-cheng |
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| Institution: | 1.College of Life Science,Inner Mongolia University/Inner Mongolia Key Laboratory of Grassland Ecology,Hohhot 010021,China;2.Maowusu Desertification Research Center, Inner Mongolia 017300,China) |
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| Abstract: | The level of genetic differentiation,gene flow among three Artemisia ordosica populations in Maowusu sandy land were analyzed using 12 pair of microsatellite primers.Similarity and genetic distance matrices were established based on the ISSR data on the basis of Nei’s and Shannon’s estimates of similarity indices and genetic distance.The results showed that: 1.The percentage of polymorphic loci was 100%,which meant that they own high genetic diversity.2.At genetic distance of 6.0,184 samples can be divided into three groups,covering the 135 sample plants,accounting for 73.34% of total sample.Combined with locating records,clustering results was in accordance with the spatial distribution,i.e.they can be divided into Artemisia ordosica population in moving sandy land,Artemisia ordosica population in fixed sandy land and Artemisia ordosica population in semi-fixed sandy land.3.There was lower gene communication between Artemisia ordosica population in moving sandy land and Artemisia ordosica population in fixed sandy land and Artemisia ordosica population in semi-fixed sandy land,0.7929 and 0.6848 respectively,there was higher gene communication between Artemisia ordosica population in fixed sandy land and Artemisia ordosica population in semi-fixed sandy land,6.2930,they own high genetic identity,93.77%;Shannon’s index in moving sandy land was low and it communicated gene with others also less,which indicated that they have sexual propagation process,but Artemisia ordosica population in moving sandy land have predominately asexual reproduction process. |
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| Keywords: | Artemisia ordosica Genetic diversity Genetic differentiation ISSR |
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