| 土壤多重抗生素抗性放线菌的筛选及其发酵产物的初步研究 |
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| 引用本文： | 王相晶,王晓舟,张继,朱兆香,向文胜,张匀华.土壤多重抗生素抗性放线菌的筛选及其发酵产物的初步研究[J].东北农业大学学报,2011,42(2):84-91. |
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| 作者姓名： | 王相晶 王晓舟 张继 朱兆香 向文胜 张匀华 |
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| 作者单位： | 王相晶,WANG Xiangjing(黑龙江省农业科学院博士后工作站,哈尔滨,150001;东北林业大学博士后流动站,哈尔滨,150086;东北农业大学生生命科学学院,哈尔滨,150030);王晓舟,张继,朱兆香,向文胜,WANG Xiaozhou,ZHANG Ji,ZHU Zhaoxiang,XIANG Wensheng(东北农业大学生生命科学学院,哈尔滨,150030);张匀华,ZHANG Yunhua(黑龙江省农业科学院博士后工作站,哈尔滨,150001) |
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| 基金项目： | 国家自然科学基金，国家科技支撑计划项目 |
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| 摘 要： | 从4个不同地区土样中共分离得到20株天然无抗菌活性或抗菌活性微弱放线菌,采用链霉素(Streptomycin)、庆大霉素(Gentamycin)及利福平(Rifampicin)三重抗性法对其进行活性菌株的筛选后,17株放线菌的发酵液显示了抑菌活性,其中1号放线菌对链霉素、庆大霉素和利福平抗性分别为40、10和150 g...
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| 关 键 词： | 放线菌 抗性筛选 发酵 抑菌活性 |
Screening of multi-antibiotic resistances actinomycetes and analysis of their metabolites |
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| WANG Xiangjing,WANG Xiaozhou,ZHANG Ji,ZHU Zhaoxiang,XIANG Wensheng,ZHANG Yunhua.Screening of multi-antibiotic resistances actinomycetes and analysis of their metabolites[J].Journal of Northeast Agricultural University,2011,42(2):84-91. |
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| Authors: | WANG Xiangjing WANG Xiaozhou ZHANG Ji ZHU Zhaoxiang XIANG Wensheng ZHANG Yunhua |
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| Institution: | 1(1. Post-doctoral Working Station of Heilongjiang Academy of Agricultural Sciences,Harbin 150001,China;2. Post-doctoral Working Station of Northeast Forestry University,Harbin 150086,China;3. College of Life Sciences,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | In this work,17 strains showed the bioactivity against tested bacteria were screened out from 20 actinomycetes with poor or without antimicrobial activity isolated from several soil samples after the treatment of three kinds of antibiotics(streptomycin,gentamicin and rifampicin) . Among these strains,No. 1 actinomycete strain exhibited the highest antimicrobial activity against Bacillus subtilis ATCC 6633 and Micrococcus luteus ATCC 9341 with a inhibition zone of 20.52 and 23.23 mm,respectively,and possessed the resistant levels of 40,10 and 150 μg·mL-1 to streptomycin,gentamicin and rifampicin,respectively. The sensitivities of the secondary metabolites to temperature and acidity-alkalinity were evaluated. The results demonstrated that antibacterial activities were almost disappeared when the supernatant was processed under 50 ℃ for 1 h,and significantly reduced after exposure to acidic or basic condition(pH<4 or >9) . However,the antibacterial activities of the mycelium extract remained almost unchanged when it was held at 90 ℃ for 1.5 h or exposed to conditions in the pH range 2-11. This actinomycete strain was identified as Streptomyces based on its morphological and physiological properties as well as 16S rDNA sequence analysis. |
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| Keywords: | actinomycete resistance screening fermentation antimicrobial activity |
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